Method for producing hematopoietic stem cells
AssigneesNational University Corporation Chiba University, Nissan Chemical Industries, Ltd.
InventorsTaito Nishino, Atsushi Iwama
Life of this patent
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Abstract
An expanding agent for hematopoietic stem cells and/or hematopoietic progenitor cells useful as a therapy for various hematopoietic diseases and useful for improvement in the efficiency of gene transfer into hematopoietic stem cells for gene therapy is provided. A method of producing hematopoietic stem cells and/or hematopoietic progenitor cells, which comprises expanding hematopoietic stem cells by culturing hematopoietic stem cells ex vivo in the presence of a compound represented by the formula following (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof (wherein R1 to R6 are as defined in the description).
Claims29
A method of producing hematopoietic stem cells and/or hematopoietic progenitor cells, the method comprising:
- expanding hematopoietic stem cells and/or hematopoietic progenitor cells by culturing hematopoietic stem cells ex vivo in the presence of a compound of formula (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof:
- wherein:
- R1 and R2 are each independently a hydrogen atom, a C1-3 alkyl group, a C1-3 alkoxy group, a hydroxyl group, a C1-3 alkylsulfonyl group, a monosaccharide residue, an oligosaccharide residue, or an amino acid residue;
- R3 is a hydrogen atom or forms a single bond together with R4;
- R4 forms a single bond together with R3 or forms a single bond together with R5;
- R5 is a hydrogen atom or forms a single bond together with R4;
- and
- R6 is a C1-10 alkyl group or a C2-10 alkenyl group.
A reagent kit, comprising an active ingredient comprising a compound of formula (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof:
- wherein:
- R1 and R2 are each independently a hydrogen atom, a C1-3 alkyl group, a C1-3 alkoxy group, a hydroxyl group, a C1-3 alkylsulfonyl group, a monosaccharide residue, an oligosaccharide residue, or an amino acid residue;
- R3 is a hydrogen atom or forms a single bond together with R4;
- R4 forms a single bond together with R3 or forms a single bond together with R5;
- R5 is a hydrogen atom or forms a single bond together with R4;
- and
- R6 is a C1-10 alkyl group or a C2-10 alkenyl group.
A method of producing transformed hematopoietic stem cells, the method comprising:
- transferring a gene into hematopoietic stem cells and/or hematopoietic progenitor cells while culturing the hematopoietic stem cells and/or hematopoietic progenitor cells ex vivo in the presence of a compound of formula (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof;
- or
- expanding hematopoietic stem cells carrying a gene transferred into them by culturing the hematopoietic stem cells ex vivo in the presence of a compound of formula (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof:
- wherein:
- R1 and R2 are each independently a hydrogen atom, a C1-3 alkyl group, a C1-3 alkoxy group, a hydroxyl group, a C1-3 alkylsulfonyl group, a monosaccharide residue, an oligosaccharide residue, or an amino acid residue;
- R3 is a hydrogen atom or forms a single bond together with R4;
- R4 forms a single bond together with R3 or forms a single bond together with R5;
- R5 is a hydrogen atom or forms a single bond together with R4;
- and
- R6 is a C1-10 alkyl group or a C2-10 alkenyl group.
An expanding agent, comprising a compound of formula (I), a tautomer or pharmaceutically acceptable salt of the compound or a solvate thereof:
- wherein:
- R1 and R2 are each independently a hydrogen atom, a C1-3 alkyl group, a C1-3 alkoxy group, a hydroxyl group, a C1-3 alkylsulfonyl group, a monosaccharide residue, an oligosaccharide residue, or an amino acid residue;
- R3 is a hydrogen atom or forms a single bond together with R4;
- R4 forms a single bond together with R3 or forms a single bond together with R5;
- R5 is a hydrogen atom or forms a single bond together with R4;
- and
- R6 is a C1-10 alkyl group or a C2-10 alkenyl group.
Dependencies are inferred from the claim text.