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US 2013/0095084 A1Published application
Methods and compositions for modulating peripheral immune function
AssigneeSanBio, Inc.
InventorsMo Dao, Casey C. Case
Life of this patent
Estimating the term…
Abstract
Disclosed herein are cell preparations useful for modulating various peripheral immune functions, methods for making said cell preparations, and methods for their use.
Claims30
A method for peripheral immunosuppression in a subject, the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
1 more element
A method for inhibiting a peripheral inflammatory response in a subject, the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
1 more element
A method for suppressing peripheral T-cell activation in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for suppressing the function of peripheral helper T-cells in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for expanding a population of peripheral regulatory T-cells (Tregs) in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for modulating peripheral production of a cytokine in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for inhibiting the differentiation of a peripheral monocyte to a dendritic cell in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for inhibiting the maturation of a peripheral dendritic cell in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for treating GVHD in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for inhibiting graft rejection in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
A method for treating a peripheral autoimmune disorder in a subject;
- the method comprising administering to the subject an effective amount of SB623 cells;
- wherein said SB623 cells are obtained by (a) providing a culture of mesenchymal stem cells;
- (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding a Notch intracellular domain (NICD) wherein said polynucleotide does not encode a full-length Notch protein;
- (c) selecting cells that comprise the polynucleotide of step (b);
- and (d) further culturing the selected cells of step (c) in the absence of selection.
2 more elements
Dependencies are inferred from the claim text.