Compositions and methods for providing hematopoietic function without hla matching
AssigneeFred Hutchinson Cancer Research Center
InventorsIrwin D. Bernstein, Colleen Delaney
Life of this patent
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Abstract
The present invention relates to methods and compositions for providing hematopoietic function in immunodeficient human patients, by selecting an expanded human umbilical cord blood stem/progenitor cell sample without taking into account the HLA-type of the expanded human cord blood stem/progenitor sample or the HLA-type of the patient; and administering the selected expanded human cord blood stem/progenitor cell sample to the patient. Methods for obtaining the expanded human cord blood stem/progenitor cell samples, banks of frozen expanded human cord blood stem/progenitor cell samples, and methods for producing such banks are also provided herein.
Claims40
A method for providing hematopoietic function to a human patient in need thereof, comprising administering an expanded human cord blood stem cell sample to the patient, wherein said administering is done without matching the HLA-type of the expanded human cord blood stem cell sample to the HLA-type of the patient, wherein the expanded human cord blood stem cell sample has been subjected to an expansion technique that has been shown to result in an at least 50-fold increase in hematopoietic stem cells or hematopoietic stem and progenitor cells in an aliquot of a human cord blood stem cell sample subjected to the expansion technique, relative to an aliquot of the human cord blood cell stem cell sample prior to being subjected to the expansion technique.
A method for providing hematopoietic function to a human patient in need thereof, comprising:
- a. selecting an expanded human cord blood stem cell sample for administration to the patient, wherein said selecting does not take into account the HLA-type of the sample or the HLA-type of the patient;
- and
- b. administering the selected sample, or an aliquot thereof, to the patient.
A method for providing hematopoietic function to a human patient in need thereof, comprising:
- (a) enriching for hematopoietic stem cells or hematopoietic stem and progenitor cells from the umbilical cord blood and/or placental blood of one or more humans at birth to produce a population enriched in hematopoietic stem cells or hematopoietic stem and progenitor cells;
- (b) expanding ex vivo the population enriched in hematopoietic stem cells or hematopoietic stem and progenitor cells to produce an expanded stem cell sample;
- and
- (c) administering the expanded stem cell sample, or an aliquot thereof, to a human patient in need of hematopoietic function, wherein said administering is done without matching the HLA type of the expanded cell sample to the HLA type of the patient.
A method of producing a bank of frozen, expanded human cord blood stem cells comprising the following steps in the order stated:
- (a) expanding, ex vivo, human cord blood stem cells present in a population enriched for hematopoietic stem cells or hematopoietic stem and progenitor cells obtained from the umbilical cord blood and/or placental blood of one or more humans at birth to produce an expanded human cord blood stem cell sample;
- (b) freezing the expanded human cord blood stem cell sample to produce a frozen expanded human cord blood stem cell sample;
- (c) storing the frozen expanded human cord blood stem cell sample;
- and
- (d) repeating steps (a)-(c) at least 50 times to produce a bank of at least 50 stored, frozen expanded human cord blood stem cell samples.
A blood bank comprising at least 50 units of frozen expanded human cord blood stem cell samples.
A computer-implemented method for selecting a frozen expanded human cord blood stem cell sample for use in providing hematopoietic function to a human patient in need thereof, comprising the following steps performed by a suitably programmed computer:
- (a) selecting an identifier from a plurality of at least 50 identifiers stored in a computer database, each identifier identifying a frozen stored expanded human cord blood stem cell sample derived from the umbilical cord blood and/or placental blood of one or more different humans at birth, wherein the selecting does not take into account the respective HLA types of the stored frozen expanded human cord blood stem cell samples corresponding to the respective identifiers, wherein the selecting is to identify a frozen stored expanded human cord blood stem cell sample for administration of the expanded human cord blood stem cell sample, or an aliquot thereof, identified by said identifier to a human patient in need thereof;
- and
- (b) outputting or displaying the selected identifier.
A computer program product for use in conjunction with a computer system, the computer program product comprising a computer readable storage medium and a computer program mechanism embedded therein, the computer program mechanism comprising:
- (a) executable instructions for selecting an identifier from a plurality of at least 50 identifiers stored in a computer database, each identifier identifying a frozen stored expanded human cord blood stem cell sample derived from the umbilical cord blood and/or placental blood of one or more humans at birth, wherein the selecting does not take into account the respective HLA types of the stored frozen expanded human cord blood stem cell samples corresponding to the respective identifiers, wherein the selecting is to identify a frozen stored expanded human cord blood stem cell sample for administration of the expanded human cord blood stem cell sample, or an aliquot thereof, identified by said identifier to a human patient in need thereof;
- and
- (b) executable instructions for outputting or displaying the selected identifier.
An apparatus comprising:
- a processor;
- a memory, coupled to the processor, the memory storing a module, the module comprising:
- (a) executable instructions for selecting an identifier from a plurality of at least 50 identifiers stored in a computer database, each identifier identifying a frozen stored expanded human cord blood stem cell sample derived from the umbilical cord blood and/or placental blood of one or more humans at birth, wherein the selecting does not take into account the respective HLA types of the stored frozen expanded human cord blood stem cell samples corresponding to the respective identifiers, wherein the selecting is to identify a frozen stored expanded human cord blood stem cell sample for administration of the expanded human cord blood stem cell sample, or an aliquot thereof, identified by said identifier to a human patient in need thereof;
- and
- (b) executable instructions for outputting or displaying the selected identifier.
Dependencies are inferred from the claim text.