Somatic Stem Cells
AssigneeStemBios Technologies, Inc.
InventorJames Wang
Life of this patent
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Abstract
A somatic stem cell that is CD10+, CXCR4+, and CD31+ and another somatic stem cell that is CD 105+, CD44+, and nestin+. Also disclosed are both a method of preparing these stem cells and a method of using them to treat degenerative diseases, e.g., a muscle-degenerative disease. The invention further includes making and using liver cells derived from the somatic cell that is CD105+, CD44+, and nestin+.
Claims21
An isolated somatic stem cell that is CD10+, CXCR4+, and CD31+.
An isolated somatic stem cell that is CD105+, CD44+, and Nestin+.
A cell bank comprising a plurality of populations of somatic stem cells, the somatic stem cells each being CD10+, CXCR4+, and CD31+ and the populations being originated from different subjects.
A cell bank comprising a plurality of populations of somatic stem cells, the somatic stem cells each being CD105+, CD44+, and Nestin+ and the populations being originated from different subjects.
A method of preparing somatic stem cells, the method comprising:
- obtaining from a subject a bodily fluid sample containing a plurality of cells,
- incubating the sample with EDTA or heparin in a container until the sample is separated into an upper layer and a lower layer,
- collecting the upper layer,
- isolating from the upper layer a population of somatic stem cells that are 0.3-6.0 micrometers in size, and
- culturing the isolated somatic stem cells in a medium containing R-Spondin-1, SCF, G-CSF, bFGF, EGF, and PDGF.
Dependencies are inferred from the claim text.