Bone morphogenic protein binding peptide
AssigneesDepartment of Veterans Affairs, The Regents of the University of California
InventorsSamuel S. Murray, Keyvan Behnam, Elsa J. Brochman-Murray
Life of this patent
Estimating the term…
Abstract
A cyclized peptide designated BMP Binding Peptide (BBP) is a synthetic peptide that avidly binds rhBMP-2. BBP increases the overall osteogenic activity of rhBMP-2, increases the rate at which rhBMP-2 induces bone formation, and BBP induces calcification alone. Compositions and substrates including BBP, and methods of using BBP are useful in therapeutic, diagnostic and clinical applications requiring calcification and osteogenesis.
Claims10
1-15. (canceled)
A method of enhancing the rate or degree of osteogenesis in vertebrate tissue, comprising applying to the tissue:
- (a) a peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis in mammalian cells;
- and
- (b) one of BMP-2 or demineralized bone matrix.
A method of inducing calcification of vertebrate tissue, comprising applying to the tissue a peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or a fragment thereof, wherein said peptide or fragment increases the degree or rate of calcification in vertebrate cells.
A method of inducing calcification of mammalian osteogenic tissue, comprising applying to the tissue a peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or a fragment thereof, wherein said peptide or fragment increases the degree or rate of calcification in mammalian chondrogenic and osteogenic precursor cells.
A method of enhancing the rate or degree of osteogenesis in vertebrate tissue, comprising:
- (a) administering osteogenic cells to the patient at a location proximate to the desired location of osteogenesis;
- (b) administering a peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or a fragment thereof, wherein said peptide or fragment increases the degree or rate of osteogenesis in mammalian cells;
- and
- (c) administering one of BMP-2 or demineralized bone matrix.
A method of enhancing the rate or degree of calcification in vertebrate tissue, comprising:
- (a) administering osteogenic cells to the patient at a location proximate to the desired location of calcification;
- (b) administering a peptide comprising the amino acid sequence of SEQ ID NO:
- 1 or a fragment thereof, wherein said peptide or fragment increases the degree or rate of calcification in vertebrate chondrogenic and osteogenic precursor cells.
21-37. (canceled)
Dependencies are inferred from the claim text.