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US 2013/0092833 A1Published application
Methods for detecting vitamin c by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsQibo Jiang, Richard E. Reitz, Sum Chan
Life of this patent
Estimating the term…
Abstract
Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.
Claims17
A method for determining the amount of vitamin C in a body fluid sample by tandem mass spectrometry, the method comprising:
- (a) purifying vitamin C from the body fluid sample by liquid chromatography;
- (b) generating a precursor ion of vitamin C having a mass/charge ratio of 175.05±0.5;
- (c) generating one or more fragment ions of the precursor ion selected from the group of fragment ions having a mass/charge ratio of 114.85±0.5, and 86.85±0.5;
- and
- (d) detecting the amount of one or more of the ions generated in step (b) or (c) or both using tandem mass spectrometry and relating the determined ions to the amount of vitamin C in the body fluid sample.
2 more elements
A method for determining the amount of vitamin C in a body fluid sample by mass spectrometry, said method comprising:
- (a) contacting a body fluid sample with a stabilizing agent to inhibit vitamin C degradation;
- (b) purifying vitamin C from the sample by high performance liquid chromatography (HPLC);
- (c) generating a precursor ion of vitamin C having a mass/charge ratio of 175.05±0.5;
- (d) generating one or more fragment ions of the precursor ion selected from the group of fragment ions having a mass/charge ratio of 114.85±0.5, and 86.85±0.5;
- and
- (e) determining the amount of one or more vitamin C ions by mass spectrometry, wherein the amount of the determined vitamin C ions is used to determine the amount of vitamin C in body fluid sample.
3 more elements
Dependencies are inferred from the claim text.