Apparatus and method for using ultrasonic radiation for controlled fragmentation of chains of nucleic acids
AssigneeMicrosonic Systems Inc.
InventorsVibhu Vivek, Babur Hadimioglu, Smriti Sharma, Kapil Dev
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Abstract
Example methods and systems are directed to controlled fragmentation of genetic samples that include chains of nucleic acid. Waveform inputs to a transducer configured as Fresnel Annular Sector Actuator (FASA) are used to focus acoustic energy at the genetic sample in a controlled fragmentation process that reduces the genetic sample to a desired average fragment size for the resulting chains of nucleic acid.
Claims38
A method of using ultrasonic radiation for controlled fragmentation of a genetic sample that includes chains of nucleic acids, the method comprising:
- providing a transducer that includes a substrate with top and bottom electrodes configured as a Fresnel Annular Sector Actuator (FASA) that directs acoustic energy in a target direction;
- providing a sample container to contain the genetic sample, the sample container being disposed in the target direction of the transducer, and the sample container being acoustically coupled to the transducer through a coupling medium;
- and
- applying an input waveform to the transducer to direct acoustic energy to the genetic sample over a specified time interval, the genetic sample being reduced to fragments of nucleic acid having an average fragment size that corresponds to the specified time interval.
An apparatus for using ultrasonic radiation for controlled fragmentation of a genetic sample that includes chains of nucleic acids, the apparatus comprising:
- a transducer that includes a substrate with top and bottom electrodes configured as a Fresnel Annular Sector Actuator (FASA) that directs acoustic energy in a target direction;
- a sample container configured to contain the genetic sample, the sample container being disposed in the target direction of the transducer, and the sample container being acoustically coupled to the transducer through a coupling medium;
- and
- a radio-frequency generator configured to apply an input waveform to the transducer to direct acoustic energy to the genetic sample over a specified time interval, the genetic sample being reduced to fragments of nucleic acid having an average fragment size that corresponds to the specified time interval.
A method of using ultrasonic radiation for controlled fragmentation of a genetic sample that includes chains of nucleic acids, the method comprising:
- accessing configuration values for a transducer that includes a substrate with top and bottom electrodes configured as a Fresnel Annular Sector Actuator (FASA) that directs acoustic energy in a target direction;
- accessing configuration values for a sample container that contains the genetic sample, the sample container being disposed in the target direction of the transducer, and the sample container being acoustically coupled to the transducer through a coupling medium;
- accessing a database for operation of the transducer with the sample container under conditions given by the configuration values for the transducer and the configuration values of the sample container, the database relating an application of an input waveform to the transducer for a specified time interval to an average fragment size for fragments of nucleic acid in a controlled fragmentation of the genetic sample;
- and
- providing an RF (radio-frequency) input to an RF generator configured to apply the input waveform to the transducer.
A non-transitory computer-readable medium that stores a computer program for using ultrasonic radiation for controlled fragmentation of a genetic sample that includes chains of nucleic acids, the computer program including instructions that, when executed by at least one computer, cause the at least one computer to perform operations comprising:
- accessing configuration values for a transducer that includes a substrate with top and bottom electrodes configured as a Fresnel Annular Sector Actuator (FASA) that directs acoustic energy in a target direction;
- accessing configuration values for a sample container that contains the genetic sample, the sample container being disposed in the target direction of the transducer, and the sample container being acoustically coupled to the transducer through a coupling medium;
- accessing a database for operation of the transducer with the sample container under conditions given by the configuration values for the transducer and the configuration values of the sample container, the database relating an application of an input waveform to the transducer for a specified time interval to an average fragment size in a controlled fragmentation of the genetic sample;
- and
- providing an RF (radio-frequency) input to an RF generator configured to apply the input waveform to the transducer.
Dependencies are inferred from the claim text.