Transformant which produces glycine repeat sequence protein
OwnerShoichi Nishioinventor
Life of this patent
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Abstract
Disclosed is a transformant in which polynucleotides comprising a nucleotide sequence encoding the amino acid sequence of all of the following proteins (1) to (3) are transfected into a microbial cell: (1) a protein that is capable of binding to FK506 and has a molecular weight of 15,000 or more and 60,000 or less; (2) a prolyl hydroxylase; and (3) a glycine repeat sequence protein having the following characteristics (A) and (B): <characteristic (A)> the polypeptide chain of the glycine repeat sequence protein having a continuous, Gly-Xaa-Yaa repeating sequence, and <characteristic (B)> an imino acid being contained in the continuous, Gly-Xaa-Yaa repeating sequence of the polypeptide chain of the glycine repeat sequence protein.
Claims22
A transformant in which all of the following polynucleotides (1), (2) and (3) are transfected into a microbial cell:
- (1) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of an FK506 binding protein that is capable of binding to FK506 and has a molecular weight of 15,000 or more and 60,000 or less;
- (2) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of a prolyl hydroxylase;
- and
- (3) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of a glycine repeat sequence protein having the following characteristics (A) and (B):
- <characteristic (A)> the polypeptide chain of the glycine repeat sequence protein having a continuous, Gly-Xaa-Yaa repeating sequence, and
- <characteristic (B)> an imino acid being contained in the continuous, Gly-Xaa-Yaa repeating sequence of the polypeptide chain of the glycine repeat sequence protein,
- wherein Xaa and Yaa each represent any amino acid.
A process for producing a glycine repeat sequence protein, comprising:
- a first step of transfecting all of the following polynucleotides (1), (2) and (3) into a microbial cell:
- (1) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of an FK506 binding protein that is capable of binding to FK506 and has a molecular weight of 15,000 or more and 60,000 or less,
- (2) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of a prolyl hydroxylase, and
- (3) a polynucleotide comprising a nucleotide sequence encoding the amino acid sequence of a glycine repeat sequence protein having the following characteristics (A) and (B):
- <characteristic (A)> the polypeptide chain of the glycine repeat sequence protein having a continuous, Gly-Xaa-Yaa repeating sequence, and
- <characteristic (B)> an imino acid being contained in the continuous, Gly-Xaa-Yaa repeating sequence of the polypeptide chain of the glycine repeat sequence protein,
- wherein Xaa and Yaa each represent any amino acid;
- a second step of culturing the transformant resulting from the first step, thereby producing the glycine repeat sequence protein;
- and
- a third step of collecting the glycine repeat sequence protein produced in the second step.
Dependencies are inferred from the claim text.