Back to results
US 2013/0075286 A1Published application
Sample carrier and method for microscopic examination of biological samples
AssigneeSiemens Aktiengesellschaft
InventorsWalter GUMBRECHT, Karsten HILTAWSKY, Daniel SICKERT
Life of this patent
Estimating the term…
Abstract
A sample carrier for microscopic examination of biological samples includes a base body and a filter membrane. The base body has at least one recess in which the filter membrane is disposed. The filter membrane makes an essentially flush closure with the surface of the sample carrier. The sample carrier has a circular shape.
Claims20
A sample carrier for microscopic examination of biological samples, the sample carrier comprising:
- a base body with at least one recess;
- and
- a filter membrane in the at least one recess, the filter membrane essentially making a flush closure with a surface of the sample carrier;
- wherein
- the sample carrier has a circular shape.
2 more elements
A method for detecting fluorescence-marked objects in a biological sample, the method comprising:
- applying the biological sample to a circular sample carrier having at least one filter membrane;
- filtering the biological sample through the filter membrane;
- treating the retentate remaining on the filter membrane with at least one fluorescence marker;
- rotating the sample carrier and scanning the sample carrier with a laser while simultaneously detecting fluorescence events with a photodetector, the frequency of the laser corresponding to an excitation frequency of an assigned fluorescence marker;
- and
- storing, upon detection of a fluorescence event, coordinates of the fluorescence event on the sample carrier.
3 more elements
Dependencies are inferred from the claim text.