Process for the production of violacein and its derivative deoxyviolacein containing bioactive pigment from chromobacterium sp. (mtcc5522)
AssigneeCouncil of Scientific and Industrial Research
InventorKrishnakumar Bhaskaran
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Abstract
The present invention discloses a process for producing purple-blue natural pigment containing violacein and its derivative (deoxyviolacein) using Chromobacterium sp. NIIST-CKK-01 (MTCC 5522, NCIM 5341; Genbank Accession No. FJ982784). The method comprises the steps of maintaining and growing the bacterium in a specific medium under defined conditions of pH, temperature and agitation. At the end of incubation, pigment and biomass is separated from the culture broth, pigment is recovered from the biomass through solvent extraction and finally pigment is concentrated by drying.
Claims10
An isolated bacterial strain, Chromobacterium sp. having accession no. MTCC 5522, wherein the said Chromobacterium cells are 0.3 to 0.6 μm wide and 0.9 to 1.5 μm long, facultative aerobic Gram negative, Oxidase positive, Indole production test negative, Methyl red test positive, Voges-Proskauer test negative, Citrate utilization test positive, TSI test glucose fermenting only, Catalase test negative and Ureas test negative.
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