Method for preparing es cells
AssigneeRiken
InventorsHaruhiko Koseki, Takanori Hasegawa, Tomoyuki Ishikura, Masashi Matsuda
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Abstract
The present invention provides a method of producing a mammalian ES cell, including cultivating a mammalian inner cell mass in a medium containing adrenocorticotropic hormone, an FGF receptor inhibitor, an MEK activation inhibitor and a GSK3 inhibitor, and isolating a mammalian ES cell from the culture.
Claims10
A method of producing a mammalian ES cell, comprising cultivating a mammalian inner cell mass in a medium containing adrenocorticotropic hormone, an FGF receptor inhibitor, an MEK activation inhibitor and a GSK3 inhibitor, and isolating a mammalian ES cell from the culture.
An isolated NOD/SCID background mouse ES cell capable of proliferation while maintaining pluripotency.
A medium containing adrenocorticotropic hormone, an FGF receptor inhibitor, an MEK activation inhibitor and a GSK3 inhibitor.
Dependencies are inferred from the claim text.