Compositions and Methods for Engineering Cells
AssigneeLife Technologies Corporation
InventorsUMA LAKSHMIPATHY, BHASKAR THYAGARAJAN, JONATHAN CHESNUT, VASILIKI ANEST, ROBERT BENNETT, PAULINE LIEU, GEORGE HANSON, DAVID THOMPSON, LUCAS CHASE, GARY SHIPLEY, ELIZABETH WILSON
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Abstract
The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.
Claims24
An isolated nucleic acid molecule comprising (a) an OriP site, (b) a DNA segment encoding the EBNA1 gene;
- (c) one or more att recombination sites;
- and (d) a DNA segment encoding at least one selectable marker.
10-11. (canceled)
The isolated nucleic acid molecule of claim 10 wherein the mammal is human.
19-64. (canceled)
66-73. (canceled)
75-92. (canceled)
94-98. (canceled)
Dependencies are inferred from the claim text.