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OwnerJosef Glössl, Richard Strasser +5inventors
InventorsJosef Glössl, Richard Strasser, Jan Mucha, Lukas Mach, Friedrich Altmann, Iain B. Wilson, Herta Steinkellner
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A DNA molecule is provided which comprises a sequence according to SEQ ID NO: 8 having an open reading frame from base pair 227 to base pair 1831 or having at least 50% homology to the above-indicated sequence, or hybridizing with the above-indicated sequence under stringent conditions, or comprising a sequence which has degenerated to the above-indicated DNA sequence because of the genetic code, the sequence coding for a plant protein having β1,2-xylosyltransferase activity or being complementary thereto.
1-32. (canceled)
A method of producing a recombinant glycoprotein, comprising expressing the recombinant glycoprotein in a plant or plant cell in which production of an endogenous β1,2-xylosyltransferase is suppressed or completely stopped, wherein said endogenous β1,2-xylosyltransferase can be identified by sequence comparison with the β1,2-xylosyltransferase sequence according to SEQ ID NO:8 with an open reading frame from base pair 227 to base pair 1831.
The method of claim 1, wherein said endogenous β1,2-xylosyltransferase is identified by sequence comparison with the β1,2-xylosyltransferase sequence according to SEQ ID NO:8 with an open reading frame from base pair 227 to base pair 1831 bp the program fastDB.
The method according to claim 1, wherein the recombinant glycoprotein is a human protein.
The method of claim 1, wherein the expression of the β1,2-xylosyltransferase is suppressed or completely blocked by a knock-out mutation of the endogenous β1,2-xylosyltransferase gene in said plant or plant cell.
The method of claim 1, wherein the expression of the β1,2-xylosyltransferase is suppressed or completely blocked by antisense inhibition in said plant or plant cell.
The method of claim 1, wherein the expression of the β1,2-xylosyltransferase is suppressed or completely blocked by transfection with a polynucleotide comprising a sequence of at least 50 nucleotides that is complementary to the sequence coding for the endogenous β1,2-xylosyltransferase.
The method of claim 1, wherein the endogenous β1,2-xylosyltransferase activity in said plant or plant cell is less than 50% of the β1,2-xylosyltransferase occurring in natural plants or plant cells without β1,2-xylosyltransferase suppression.
A method of producing a recombinant glycoprotein, comprising:
A method of producing a plant or plant cell with reduced or no endogenous β1,2-xylosyltransferase activity, comprising:
Dependencies are inferred from the claim text.