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US 2012/0322733 A1Published application
Novel peptidyl alpha-hydroxyglycine alpha-amidating lyases
AssigneeNovo Nordisk A/S
InventorAllan C. Shaw
Life of this patent
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Abstract
The present patent application concerns an enzyme capable of catalysing the conversion of a α-hydroxyglycine to an α-amide and the use of such enzymes for producing a C-terminal α-amidated peptide.
Claims14
An isolated polypeptide comprising an amino acid sequence comprising the following motif 1:
- Xaa1 Val Xaa2 Asp Arg Xaa3 Xaa4 Xaa5 Arg Xaa6 Gln Xaa7 Xaa8 Xaa9 Xaa10 Xaa11 Gly Xaa12 Xaa13 Xaa14 Xaa15 Xaa16 Trp;
- where Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, Xaa6, Xaa7, Xaa8, Xaa9, Xaa10, Xaa11 Xaa12, Xaa13. Xaa14, Xaa15, and Xaa16 are selected independently from a natural occurring amino acid, provided that Xaa1 and Xaa7 are not Cys,
- wherein the polypeptide is capable of catalysing the conversion of a α-hydroxyglycine to an α-amide.
9-10. (canceled)
(canceled)
Dependencies are inferred from the claim text.