Immunoglobulin reduced in thrombogenic agents and preparation thereof
AssigneeOmrix Biopharmaceuticals Ltd.
InventorsRoni MINTZ, Oleg Belyaev, Israel Nur, Liliana Bar, Roberto Meildler
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Abstract
The invention relates to an immunoglobulin composition reduced in thrombogenic agents and to methods for its preparation. One method comprises subjecting an immunoglobulin containing solution to a negative cation exchanger chromatography at a pH in the range of higher than 3.8 to equal to or lower than 5.3. The solution can also be subjected to a negative anion exchanger chromatography at a pH in the range of 7 to 8.2.
Claims52
A method for removing a thrombogenic agent from an immunoglobulin containing solution, the method comprising the steps of:
- providing the immunoglobulin containing solution at a pH in the range of higher than 3.8 to equal to or lower than 5.3;
- providing a support comprising immobilized negatively charged groups;
- contacting the solution with the support;
- and collecting an unbound fraction I.
A method for preparing an immunoglobulin composition comprising the steps of:
- subjecting an immunoglobulin containing solution to at least two steps of negative chromatography:
- an anion exchanger chromatography at a pH in the range of 7 to 8.2;
- and a cation exchanger chromatography at a pH in the range of higher than 3.8 to equal to or lower than 5.3.
Dependencies are inferred from the claim text.