Method and Apparatus for Conducting an Assay
AssigneePyrobett Pte Ltd.
InventorsJohn Corbett, John Corbett, SR.
Life of this patent
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AssigneePyrobett Pte Ltd.
InventorsJohn Corbett, John Corbett, SR.
Estimating the term…
The present invention relates to methods and apparatus for conducting an assay. In particular, the present invention relates to a rotatable platform which can be used for conducting an assay, in particular multi-step assays. The present invention provides a rotatable platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform, or to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform. The invention also relates to methods, apparatus, a kit and the use of the rotatable platform for conducting an assay. In particular, the invention has been developed primarily for use in sequencing nucleic acid by pyrosequencing, however the invention is not limited to this field.
A rotatable platform for conducting an assay, said platform adapted to immobilise a first binding partner in one or more discrete areas on a surface of said platform.
A rotatable platform according to any one of the preceding claims wherein said platform is formed of a plastics material, selected from the group consisting of polycarbonate, polystyrene, high impact polystyrene, polyethylene and polypropylene, or is formed from glass or quartz.
A rotatable platform according to any one of the preceding claims wherein said one or more discrete areas are a coating on said platform, wherein said coating is adapted to immobilise said first binding partner.
A rotatable platform according to any one of the preceding claims wherein said first binding partner is chemically adsorbed or covalently or ionically, or hydrogen bonded onto said surface of said platform, or van der Waals forces immobilise said first binding partner to said surface of said platform.
A rotatable platform according to any one of the preceding claims further comprising a trough disposed at the periphery of said platform for receiving waste fluids which are spun off or centrifuged away from the surface of said rotatable platform during its rotation.
A rotatable platform for conducting an assay, said platform adapted to selectively immobilise a second binding partner in one or more discrete areas on a surface of said platform.
A rotatable platform for conducting sequencing of a nucleic acid strand, said platform adapted to immobilise a nucleic acid strand binding partner in one or more discrete areas on a surface of said platform.
A rotatable platform for conducting sequencing of a nucleic acid strand, said platform adapted to selectively immobilise a nucleic acid strand in one or more discrete areas on a surface of said platform.
Dependencies are inferred from the claim text.