US 2012/0278910 A1 · Transgenic Pig in which HO-1 and TNFR1-Fc are Simultaneously Expressed, and Method for Producing Same

Abstract

The present invention relates to a method for producing a transgenic pig in which immune rejection response is inhibited, and in which human HO-1 genes and TNFR1-Fe fusion genes are simultaneously expressed. The present invention also relates to a transgenic pig for organ transplantation, which is produced by the method, and in which immune rejection response is inhibited. The present invention also relates to a somatic-cell-donating cell strain for producing the transgenic pig, and to a method for producing organs, from the transgenic pig, in which the immune rejection response is inhibited.

Claims12

Dependent claims

A method for producing a transgenic pig suppressed in immune rejection response, comprising:

a) isolating somatic cells from a pig;
b) introducing a gene coding for human HO-1 (Heme oxygenase-1) protein and a gene coding for human TNFR1-Fc (Tumor necrosis factor receptor 1-Fc) fusion protein into the somatic cells;
c) selecting and culturing the somatic cells that are introduced with the genes;
d) removing the nucleus from an oocyte of the pig and fusing it with the selected somatic cell to prepare a somatic cell nuclear transferred embryo;
and
e) implanting the embryo.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/520,056Filed 21 Dec 2010

Priority

KR 10-2009-013454830 Dec 2009

Published

1 Nov 2012

Term ends

Not estimatedThe source records don’t support an estimate.

Classification16 codes

C07K 14/7151
For cytokines; for lymphokines; for interferons: for tumor necrosis factor [TNF], for lymphotoxin [LT]
A01K 67/0275
New or modified breeds of vertebrates: genetically modified vertebrates, e.g. transgenic
C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12N 15/8509
For animal cells: for producing genetically modified animals, e.g. transgenic

Transgenic Pig in which HO-1 and TNFR1-Fc are Simultaneously Expressed, and Method for Producing Same

Life of this patent

Transgenic Pig in which HO-1 and TNFR1-Fc are Simultaneously Expressed, and Method for Producing Same

Life of this patent

Abstract

Claims12