Loading patent…
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
Estimating the term…
The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.
1-10. (canceled)
A method for the preparation of 2-hydroxyisobutyric acid (2-HIBA) comprising the successive steps allowing conversion of acetyl-CoA into 2-hydroxyisobutyric acid, said successive steps consisting in:
A microorganism modified for an improved production of 2-hydroxyisobutyric acid, wherein said microorganism expresses the genes coding for the enzymes having the enzymatic activities necessary for the following conversions a) converting acetyl-CoA into 3-hydroxybutyryl-CoA b) converting 3-hydroxybutyryl-CoA previously obtained into 2-hydroxyisobutyryl-CoA, and c) converting 2-hydroxyisobutyryl-CoA into 2-hydroxyisobutyric acid.
Dependencies are inferred from the claim text.