Microfluidic system and method for automated processing of particles from biological fluid

The microfluidic system of claim 9, wherein the binding agents selectively bind to abundant proteins comprising at least one protein selected from the group consisting of:

• albumin, IgG, IgA, IgM, fibrinogen, haptoglobin, alpha 1 antitrypsin, Apo A I, Apo A II, and A2 macroglobulin.

A microfluidic system for automatically depleting particles not of interest from a sample of biological fluid comprising particles of interest and particles not of interest, wherein the particles of interest include at least one of proteins and nucleic acids, the system comprising:

• a sampling module configured to receive the sample of biological fluid;
• a first microfluidic particle depletion module, fluidically coupled to the sampling module to receive the sample and configured to subject the received sample to a force that separates at least a portion of the particles not of interest from the sample, thereby providing a depleted sample;
• a second microfluidic particle depletion module, fluidically coupled to the first microfluidic particle depletion module to receive the depleted sample, comprising a microfluidic chamber and binding agents disposed within the microfluidic chamber, wherein the binding agents are configured to selectively bind to at least one of proteins of interest and proteins not of interest.