Probe for detecting polymorphism in cyp3a gene, method of detecting polymorphism, method of evaluating drug efficacy, and reagent kit for detecting polymorphism

A probe comprising one oligonucleotide selected from the group consisting of oligonucleotides P1, P1′, P2, P2′, P3, P3′, P4, P4′, P5, and P5′, wherein

• P1 is an oligonucleotide having a sequence including the 401st to the 411th bases of SEQ ID NO:
• 1 and having a length of from 11 bases to 50 bases, the sequence having at least 85% identity to the 401st to the 411th bases of SEQ ID NO:
• 1 with the exception that the base corresponding to the 411th base in SEQ ID NO:
• 1 is cytosine411th;
• P1′ is an oligonucleotide having a sequence including the 401st to the 411th bases of SEQ ID NO:
• 1 and having a length of from 11 bases to 50 bases, the sequence being hybridized under stringent conditions to a complementary strand of the 401st to the 411th bases of SEQ ID NO:
• 1 with the exception that the base corresponding to the 411th base in SEQ ID NO:
• 1 is cytosine411th;
• P2 is an oligonucleotide having a sequence including the 201st to the 205th bases of SEQ ID NO:
• 2 and having a length of from 5 bases to 50 bases, the sequence having at least 85% identity to the 201st to the 205th bases of SEQ ID NO:
• 2 with the exception that the base corresponding to the 205th base in SEQ ID NO:
• 2 is cytosine;
• P2′ is an oligonucleotide having a sequence including the 201st to the 205th bases of SEQ ID NO:
• 2 and having a length of from 5 bases to 50 bases, the sequence being hybridized under stringent conditions to a complementary strand of the 201st to the 205th bases of SEQ ID NO:
• 2 with the exception that the base corresponding to the 205th base in SEQ ID NO:
• 2 is cytosine;
• P3 is an oligonucleotide having a sequence complementary to a sequence including the 190th to the 201st bases of SEQ ID NO:
• 2 and having a length of from 12 bases to 50 bases, the sequence having at least 85% identity to the 190th to the 201st bases of a complementary strand of SEQ ID NO:
• 2 with the exception that the base complementary to the 190th base in SEQ ID NO:
• 2 is cytosine;
• P3′ is an oligonucleotide having a sequence complementary to a sequence including the 190th to the 201st bases of SEQ ID NO:
• 2 and having a length of from 12 bases to 50 bases, the sequence being hybridized under stringent conditions to the 190th to the 201st bases of SEQ ID NO:
• 2 with the exception that the base complementary to the 190th base in SEQ ID NO:
• 2 is cytosine;
• P4 is an oligonucleotide having a sequence complementary to a sequence including the 186th to the 201st bases of SEQ ID NO:
• 2 and having a length of from 16 bases to 50 bases, the sequence having at least 85% identity to the 186th to the 201st bases of a complementary strand of SEQ ID NO:
• 2 with the exception that the base complementary to the 186th base in SEQ ID NO:
• 2 is cytosine;
• P4′ is an oligonucleotide having a sequence complementary to a sequence including the 186th to the 201st bases of SEQ ID NO:
• 2 and having a length of from 16 bases to 50 bases, the sequence being hybridized under stringent conditions to the 186th to the 201st bases of SEQ ID NO:
• 2 with the exception that the base complementary to the 186th base in SEQ ID NO:
• 2 is cytosine;
• P5 is an oligonucleotide having a sequence complementary to a sequence including the 496th to the 501st bases of SEQ ID NO:
• 3 and having a length of from 6 bases to 50 bases, the sequence having at least 85% identity to the 496th to the 501st bases of a complementary strand of SEQ ID NO:
• 3 with the exception that the base complementary to the 496th base in SEQ ID NO:
• 3 is cytosine;
• and
• P5′ is an oligonucleotide having a sequence complementary to a sequence including the 496th to the 501st bases of SEQ ID NO:
• 3 and having a length of from 6 bases to 50 bases, the sequence being hybridized under stringent conditions to the 496th to the 501st bases of SEQ ID NO:
• 3 with the exception that the base complementary to the 496th base in SEQ ID NO:
• 3 is cytosine.