Screening method for cell aging
AssigneeChronos Therapeutics Limited
InventorsElizabeth Jane Mellor, Michael Youdell, Anitha Nair, Alexandre Akoulitchev
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Abstract
The present invention relates to a method for increasing the chronological lifespan of a cell comprising disrupting the function of at least one of the SAGA1 SLIK and/or SALSA complexes in said cell.
Claims24
A method for increasing a chronological lifespan of a cell comprising disrupting a function of at least one of a SAGA, SLIK or SALSA complex in said cell.
A method for identifying a potential modulator of a chronological life span (CLS) of a cell, comprising the steps of
- i) contacting a cell having a known Histone 3 Lysine 18 (H3K18) acetylation status with a compound;
- and
- ii) determining if said compound has an effect on the acetylation status of H3K18 in said cell;
- wherein, a change in the acetylation status of H3K18 in the cell indicates that the compound modulates CLS.
A method for identifying a replication status of a cell comprising identifying an acetylation state of H3K18 in said cell, wherein a presence of acetyl modification of H3K18 indicates that the cell is an actively replicating cell and an absence of acetyl modification of H3K18 indicates the cell is no longer replicating.
A method of identifying a change in a CLS of a cell comprising identifying an acetylation state of H3K18 in the cell and comparing the acetylation state of H3K18 in the cell to the acetylation state of a control cell, wherein loss of H3K18Ac when compared to the control cell indicates an increased CLS and acquisition of H3K18Ac indicates a reduced CLS.
A method of diagnosing a disorder associated with a change in a CLS of a cell, said method comprising identifying an acetylation status of H3K18 of a cell previously isolated from a subject and comparing said acetylation status to an acetylation status of a control cell.
Dependencies are inferred from the claim text.