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AssigneeNovartis Vaccines and Diagnostics, Inc.
InventorsSusan W. BARNETT, Jan ZUR MEGEDE, Indresh SRIVASTAVA, Ying LIAN, Karin HARTOG, Hong LIU, Catherine GREER, Mark SELBY, Christopher WALKER
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The present invention relates to the efficient expression of HIV polypeptides in a variety of cell types, including, but not limited to, mammalian, insect, and plant cells. Synthetic expression cassettes encoding the HIV Gag-containing polypeptides are described, as are uses of the expression cassettes in applications including DNA immunization, generation of packaging cell lines, and production of Env-, tat- or Gag-containing proteins. The invention provides methods of producing Virus-Like Particles (VLPs), as well as, uses of the VLPs including, but not limited to, vehicles for the presentation of antigens and stimulation of immune response in subjects to whom the VLPs are administered.
An expression cassette comprising a first polynucleotide sequence and a second polynucleotide sequence, wherein the first polynucleotide sequence encodes an HIV Gag polypeptide and has at least 90% sequence identity to SEQ ID NO:20, and the second polynucleotide sequence encodes an HIV Env polypeptide.
An expression cassette, comprising a first polynucleotide sequence that encodes an HIV Gag polypeptide, a second polynucleotide sequence encodes an HIV Env polypeptide, and a third polynucleotide that encodes an HIV protease polypeptide.
Dependencies are inferred from the claim text.