Enhancement of regeneration by modulation of notum activity
AssigneeWhitehead Institute for Biomedical Research
InventorsPeter Reddien, Christian Petersen
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Abstract
The invention provides compositions and methods of use for identifying modulators of NOTUM, e.g., NOTUM inhibitors. In some aspects, identified compounds are useful for modulating Wnt signaling at sites of tissue damage. The invention further provides methods of promoting regeneration by inhibiting NOTUM.
Claims35
A method of enhancing regeneration in a subject, the method comprising inhibiting NOTUM in the subject.
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A method of upregulating Wnt signaling at a site of tissue damage in a subject, the method comprising inhibiting NOTUM in the subject.
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A cell comprising a heterologous nucleic acid that encodes a fusion protein comprising:
- (a) a region comprising a GPI anchor attachment sequence of a vertebrate glypican;
- and (b) a region comprising a detectable polypeptide.
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A composition comprising:
- (a) an isolated NOTUM polypeptide;
- (b) a reporter molecule comprising a GPI anchor and a detectable moiety;
- and (c) a test compound.
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A method of identifying a compound that upregulates Wnt signaling at sites of tissue damage, the method comprising identifying a compound that inhibits NOTUM enzymatic activity.
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A method of identifying a compound that enhances regeneration, the method comprising identifying a compound that inhibits NOTUM enzymatic activity.
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Dependencies are inferred from the claim text.