Cytokine compositions and methods of use thereof
OwnerK. Christopher Garcia, Patrick Lupardus +2inventors
InventorsK. Christopher Garcia, Patrick Lupardus, Sashank K. Reddy, Gregory J. Sieczkiewicz
Life of this patent
Estimating the term…
Abstract
Polypeptides, including non-naturally occurring and recombinantly modified polypeptides related to the p19 subunit of IL-23, methods of making such molecules and methods of using such molecules as therapeutic, prophylactic and diagnostic agents are provided.
Claims79
An isolated protein comprising a polypeptide sequence at least 90% identical to SEQ ID NO:1, wherein at least one residue selected from the group consisting of residues 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, and 152 is mutated or deleted.
The protein of any preceding claim wherein the at least one amino acid is mutated to a non-conserved residue.
An isolated protein comprising a polypeptide sequence at least 90% identical to SEQ ID NO:1, wherein at least one residue selected from the group consisting of residues 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46 or 47 is mutated or deleted.
The protein of any preceding claim further comprising an IL-23 p40 subunit bound to the isolated polypeptide to form a complex.
The protein of any preceding claim further comprising an Fc domain.
The protein of any preceding claim further comprising a signal sequence.
A nucleic acid comprising a sequence encoding the protein of any preceding claim.
An isolated protein comprising an immunoglobulin heavy chain variable domain and/or an immunoglobulin light chain variable domain, wherein one or both of the variable domains form an antigen binding site that binds to IL-23 p19 at an epitope comprising one or more of amino acids 29-47 or 141-152.
An isolated protein comprising an isolated polypeptide comprising a sequence at least 90% identical to SEQ ID NO:1, wherein at least one amino acid selected from the group consisting of amino acids 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, or 27 is mutated or deleted.
A protein comprising an isolated polypeptide comprising a sequence at least 90% identical to SEQ ID NO:
- 1, wherein at least one amino acid selected from the group consisting of amino acids 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, or 117 is mutated or deleted.
The protein of any preceding claim wherein no more than five amino acids are mutated from SEQ ID NO:
- 1.
The protein of any preceding claim that is identical to SEQ ID NO:
- 1 in the region of 1-9, 28-100, and 118-170.
The protein of any preceding claim further comprising an IL-23 p40 subunit bound to the isolated polypeptide to form a complex.
An isolated protein comprising an immunoglobulin heavy chain variable domain and/or an immunoglobulin light chain variable domain, wherein one or both of the variable domains form an antigen binding site that binds to IL-23 p19 at an epitope comprising one or more of amino acids 10-27.
An isolated protein comprising an immunoglobulin heavy chain variable domain and/or an immunoglobulin light chain variable domain, wherein one or both of the variable domains form an antigen binding site that binds to IL-23 p19 at an epitope comprising one or more of amino acids 101-107 or 108-117.
Dependencies are inferred from the claim text.