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US 2012/0269734 A1Published application
Self-Illuminating Dot Systems and Methods of Use Thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJianghong Rao, Min-kyung So, Chenjie Xu, Andreas M. Loening, Sanjiv S. Gambhir
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Abstract
Generally, aspects of the present disclosure are directed to conjugate systems, self-illuminating quantum dot conjugates, methods of detecting a target in a host, methods of treating a disease in a host, and the like.
Claims43
A conjugate system, comprising:
- a self-illuminating quantum dot conjugate and a bioluminescence initiating compound, wherein the self-illuminating quantum dot conjugate includes a bioluminescence donor molecule and a quantum dot, wherein the bioluminescence donor molecule and the bioluminescence initiating compound interact to produce a bioluminescence energy, and wherein the quantum dot emits a fluorescence energy in response to the non-radiative transfer of the bioluminescence energy from the bioluminescence donor molecule to the quantum dot.
A conjugate system, comprising:
- a bioluminescence donor molecule and a quantum dot, wherein a bioluminescence energy is produced from the bioluminescence donor molecule, and wherein the quantum dot emits a fluorescence energy in response to the non-radiative transfer of the bioluminescence energy from the bioluminescence donor molecule to the quantum dot.
A method of detecting a target in a host, comprising:
- providing a self-illuminating quantum dot conjugate, wherein the self-illuminating quantum dot conjugate includes a bioluminescence donor molecule, a quantum dot, and at least one agent, wherein the agent has an affinity for the target;
- introducing the self-illuminating quantum dot conjugate to a host;
- introducing a bioluminescence initiating compound to the host;
- and
- determining the presence and location of the target in the host corresponding to the agent by detecting the self-illuminating quantum dot conjugate upon interaction with the bioluminescence initiating compound.
2 more elements
A method of treating a disease in a host, comprising:
- providing a self-illuminating quantum dot conjugate, wherein the self-illuminating quantum dot conjugate includes a bioluminescence donor molecule, a quantum dot, and a first agent, wherein the first agent is effective at treating the disease;
- introducing the self-illuminating quantum dot conjugate to the subject in need of treatment of the disease;
- introducing a bioluminescence initiating compound to the host;
- and
- determining the location of the self-illuminating quantum dot conjugate in the host upon interaction with the bioluminescence initiating compound.
2 more elements
A method of detecting a target in a host, comprising:
- providing a self-illuminating quantum dot conjugate, wherein the self-illuminating quantum dot conjugate includes a bioluminescence donor molecule, a quantum dot, and a first agent, wherein the first agent is disposed between the quantum dot and the bioluminescence donor molecule, and wherein the first agent is degraded by the target;
- introducing the self-illuminating quantum dot conjugate to a host;
- introducing a bioluminescence initiating compound to the host;
- detecting a fluorescent energy emitted from the quantum dot;
- and
- determining the presence of the target in the host by detecting decay of the fluorescent energy emitted from the quantum dot of the self-illuminating quantum dot conjugate upon degradation of the first agent.
3 more elements
A method of detecting a target in a system, comprising:
- providing a bioluminescence donor molecule complex and a quantum dot complex, wherein the bioluminescence donor molecule complex includes a bioluminescence donor molecule, wherein the quantum dot complex includes a quantum dot;
- introducing each of the bioluminescence donor molecule complex and the quantum dot complex independently to the system, wherein if the system includes the target, the target interacts with at least one of the bioluminescence donor molecule complex or the quantum dot complex to cause the bioluminescence donor molecule complex and the quantum dot complex to become substantially close to one another;
- introducing a bioluminescence initiating compound to the system, wherein the bioluminescence donor molecule and the bioluminescence initiating compound interact to produce a bioluminescence energy, and wherein the quantum dot emits a fluorescence energy in response to non-radiative transfer of the bioluminescence energy from the bioluminescence donor molecule to the quantum dot;
- and
- determining the presence of the target by detecting the fluorescent energy emitted from the quantum dot.
2 more elements
A method of detecting a target in a system, comprising:
- providing a bioluminescence donor molecule complex and a quantum dot complex, wherein the bioluminescence donor molecule complex includes a bioluminescence donor molecule, wherein the quantum dot complex includes a quantum dot, wherein one of the bioluminescence donor molecule complex or the quantum dot complex includes a first agent that has an affinity for the first target, wherein the first target undergoes a change to a second target upon interaction with the first agent, wherein the other of the bioluminescence donor molecule complex or the quantum dot complex includes a second agent that has an affinity for the second target;
- introducing each of the bioluminescence donor molecule complex and the quantum dot complex independently to the system, wherein if the system includes the first target the first target undergoes a change to the second target upon interaction with the first agent, wherein the bioluminescence donor molecule complex and the quantum dot complex become substantially close to one another upon interaction of the third agent with the second target;
- introducing a bioluminescence initiating compound to the system, wherein the bioluminescence donor molecule and the bioluminescence initiating compound interact to produce a bioluminescence energy, and wherein the quantum dot emits a fluorescence energy in response to the non-radiative transfer of the bioluminescence energy from the bioluminescence donor molecule to the quantum dot;
- and
- determining the presence of the second target by detecting the fluorescent energy emitted from the quantum dot.
2 more elements
A method of detecting a target in a system, comprising:
- providing a bioluminescence donor molecule complex and a quantum dot complex, wherein the bioluminescence donor molecule complex includes a bioluminescence donor molecule, wherein the quantum dot complex includes a quantum dot, wherein one of the bioluminescence donor molecule complex or the quantum dot complex includes a first agent that has an affinity for the first target, wherein the first agent undergoes a change to a second agent upon interaction with the first target, wherein the other of the bioluminescence donor molecule complex or the quantum dot complex includes a third agent that has an affinity for the second target;
- introducing each of the bioluminescence donor molecule complex and the quantum dot complex independently to the system, wherein if the system includes the first target the first agent undergoes a change to the second agent upon interaction with the first agent, wherein the bioluminescence donor molecule complex and the quantum dot complex become substantially close to one another upon interaction of the third agent with the second agent;
- introducing a bioluminescence initiating compound to the system, wherein the bioluminescence donor molecule and the bioluminescence initiating compound interact to produce a bioluminescence energy, and wherein the quantum dot emits a fluorescence energy in response to the non-radiative transfer of the bioluminescence energy from the bioluminescence donor molecule to the quantum dot;
- and
- determining the presence of the second target by detecting the fluorescent energy emitted from the quantum dot.
2 more elements
Dependencies are inferred from the claim text.