Methods and compositions for detecting one or more target agents using tracking components
AssigneeAntara BioSciences Inc.
InventorsI-Min M. Jen, Mark O. Trulson, Kilian Dill, Marc R. Labgold, Dawei Sheng, George G. Jokhadze, Chandramohan V. Ammini, Peter Lobban
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Abstract
The present invention provides devices and methods for real time detection of target agents in a sample. These devices utilize tracking technology and selective binding to allow the identification of one or more target agents in a sample, and preferably in a biological sample. The present invention provides specific embodiments employing radio frequency identification devices.
Claims188
A method of determining a presence of a target agent in a sample comprising:
- (a) mixing said sample with tracking complexes comprising a tracking component conjugated to a capture moiety specific for said target agent, thereby producing a first mixture comprising reacted complexes, which are those of said tracking complexes that are associated with said target agent, and unreacted complexes, which are those of said tracking complexes that are not associated with said target agent;
- (b) separating said reacted complexes from said unreacted complexes by contacting said first mixture with immobilized binding partners, wherein said immobilized binding partners facilitate separation of said unreacted complexes from said reacted complexes to produce a second mixture comprising said unreacted complexes and a third mixture comprising said reacted complexes;
- and
- (c) detecting a presence of said reacted complexes by detecting a signal of said tracking component in said third mixture, which is indicative of said presence of said target agent in said sample.
A method of determining a presence of a target agent in a sample comprising:
- (a) introducing said sample to a matrix comprising one or more immobilized binding partners that specifically bind to said target agent, thereby facilitating formation of an immobilized complex comprising said target agent and one of said one or more immobilized binding partners;
- (b) contacting said immobilized complex with tracking complexes comprising a tracking component conjugated to a capture moiety that interacts with said immobilized complex, thereby producing a mixture comprising reacted complexes, which are those of said tracking complexes that are associated with said immobilized complex, and unreacted complexes, which are those of said tracking complexes that are not associated with said immobilized complex, wherein said reacted complexes are in an immobilized phase and said unreacted complexes are in a solution phase;
- (c) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (d) operating said detection device to interrogate said immobilized phase;
- and
- (e) detecting said signal, wherein said signal is indicative of said presence of said tracking component in said immobilized phase, which is indicative of said presence of said target agent in said sample.
A method of determining a presence of a target nucleic acid in a sample comprising:
- (a) mixing said sample with a capture oligo to create a first mixture comprising hybridized complexes, which are those of said capture oligos that are associated with said target nucleic acid;
- (b) adding tracking complexes to said first mixture, wherein each of said tracking complexes comprises a tracking component conjugated to a capture moiety specific for said hybridized complexes, thereby producing a second mixture comprising reacted complexes, which are those of said tracking complexes that are associated with said hybridized complexes, and unreacted complexes, which are those of said tracking complexes that are not associated with said hybridized complexes;
- (c) isolating said reacted complexes from said unreacted complexes;
- (d) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (e) operating said detection device to interrogate said reacted complexes subsequent to said isolating of step (c);
- and
- (f) detecting said signal, wherein said signal is indicative of said presence of said target agent in said sample.
A method of determining a presence of a target agent in a sample comprising:
- (a) labeling components of said sample including said target agent to create a first mixture comprising labeled target agent;
- (b) mixing said first mixture with tracking complexes comprising a tracking component conjugated to a capture moiety that interacts with said labeled target agent, thereby producing a second mixture comprising reacted complexes, which are tracking complexes that are associated with said labeled target agent, and unreacted complexes, which are tracking complexes that are not associated with said labeled target agent;
- (c) separating said unreacted complexes from said reacted complexes to produce a third mixture comprising said unreacted complexes and a fourth mixture comprising said reacted complexes;
- (d) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (e) operating said detection device to interrogate said fourth mixture;
- and
- (f) detecting said signal, wherein said signal is indicative of said presence of said tracking component in said fourth mixture, which is indicative of said presence of said target agent in said sample.
The method of claim 81, wherein said tracking component is an RFID tag.
The method of claim 83, wherein said labeled target agent is labeled with a magnetic label.
A method of determining a presence of a target agent in a sample comprising:
- (a) labeling components from said sample with tracking components to create a first mixture comprising a tracking component-target agent complex;
- (b) contacting said first mixture with immobilized binding partners, wherein said immobilized binding partners facilitate immobilization of said tracking component-target agent complex to produce a second mixture comprising immobilized tracking component-target agent complex and a third mixture comprising any of said tracking components that did not form said tracking component-target agent complex;
- (c) separating said second mixture from said third mixture;
- (d) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (e) operating said detection device to interrogate said second mixture;
- and
- (f) detecting said signal, wherein said signal is indicative of said presence of said one of said tracking components in said second mixture, which is indicative of said presence of said target agent in said sample.
The method of claim 87, wherein said tracking components are RFID tags.
The method of claim 87, wherein said immobilized binding partners are immobilized on a matrix that is a component of said detection device.
The method of claim 87, further comprising determining a presence of a plurality of target agents, wherein said immobilized binding partners comprise a set of immobilized binding partners, wherein each of said set is at a known location on a matrix, and further wherein each of said set specifically associates with only one of said plurality of target agents, and further wherein said detecting further comprises determining a signal location on said matrix from which said signal is generated and correlating said signal location to which of said set of immobilized binding partners is known to be at said signal location on said matrix, thereby identifying which of said plurality of target agents is in said sample.
A method of detecting binding between a target agent and a capture moiety, comprising:
- (a) immobilizing said target agent to produce an immobilized target agent;
- (b) introducing a tracking complex to said immobilized target agent, wherein said tracking complex comprises a tracking component and a capture moiety, wherein said capture moiety specifically interacts with said immobilized target agent, thereby producing
- (c) an immobilized phase comprising reacted complexes, which are those of said tracking complexes that are associated with said immobilized target agent, and
- (d) a solution phase comprising unreacted complexes, which are those of said tracking complexes that are not associated with said target agent;
- (e) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (f) operating said detection device to interrogate said immobilized phase;
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- (g) detecting said signal, wherein said signal is indicative of said presence of said tracking component in said immobilized phase, which is indicative of said binding between said target agent and said capture moiety.
A composition comprising:
- (a) a matrix;
- (b) an immobilized binding partner associated with said matrix;
- (c) a target agent associated with said immobilized binding partner;
- and
- (d) a tracking component associated with said target agent.
A system for determining a presence of a target agent in a sample comprising:
- (a) a sample containing said target agent;
- (b) tracking components associated with said target agent;
- and
- (c) a detection device capable of detecting said tracking components associated with said target agent.
A diagnostic tool for detecting a target agent in a sample, comprising:
- (a) a capture moiety which binds preferentially to a target agent to form a target agent-capture moiety complex;
- (b) a tracking component that can be associated with said target agent-capture moiety complex;
- and
- (c) a detection device.
A method of doing business, said method comprising use of a signal related to a presence of a tracking component to determine appropriate medical intervention for a patient, said method comprising:
- (a) obtaining a sample from said patient whereby a target agent may be present in said sample;
- (b) mixing said sample with tracking complexes comprising said tracking component conjugated to a capture moiety specific for said target agent, thereby producing a first mixture comprising reacted complexes, which are those of said tracking complexes that are associated with said target agent, and unreacted complexes, which are those of said tracking complexes that are not associated with said target agent;
- (c) contacting said first mixture with immobilized, binding partners, wherein said immobilized binding partners facilitate separation of said unreacted complexes from said reacted complexes to produce a second mixture comprising said unreacted complexes and a third mixture comprising said reacted complexes
- (d) providing a detection device comprising a reader, wherein said reader detects said signal based on a presence of said tracking component;
- (e) operating said detection device to interrogate said third mixture;
- and
- (f) detecting said signal, wherein said signal is indicative of said presence of said tracking component in said third mixture, which is indicative of said presence of said target agent in said sample.
A radio frequency signal used to determine appropriate medical intervention for a patient, whereby said radio frequency signal is indicative of a presence of a target agent in a sample taken from said patient.
A method of determining a presence of a target agent in a sample wherein said target agent is not contacted with a detection device, comprising:
- (a) mixing said sample with tracking complexes comprising a tracking component conjugated to a capture moiety specific for said target agent, thereby producing a first mixture comprising reacted complexes, which are those of said tracking complexes that are associated with said target agent, and unreacted complexes, which are those of said tracking complexes that are not associated with said target agent;
- (b) separating said target agent from said tracking components in said reacted complexes to produce a second mixture comprising said target agent and a third mixture comprising said tracking components;
- (c) providing a detection device comprising a reader, wherein said reader detects a signal based on a presence of said tracking component;
- (d) operating said detection device to interrogate said third mixture;
- and
- (e) detecting said signal, wherein said signal is indicative of said presence of said tracking component in said third mixture, which is indicative of said presence of said target agent in said sample.
A method of determining a presence of a target nucleic acid in a sample comprising:
- (a) mixing a first complex comprising a tracking component capable of generating a signal, and a first nucleic acid with a sample suspected of containing a target nucleic acid capable of forming a nucleic acid duplex with said first nucleic acid to form a second complex;
- (b) contacting said second complex with a moiety capable of associating with said duplex, wherein said moiety is capable of effecting said signal;
- and
- (c) determining said presence of said target nucleic acid by detecting an effect imparted by said moiety on said signal.
A method of determining a sequence of a target nucleic acid comprising:
- (a) mixing a first complex comprising a tracking component capable of generating a signal, and a first nucleic acid with a sample suspected of containing a target nucleic acid capable of forming a nucleic acid duplex with said first nucleic acid to form a second complex;
- (b) contacting said second complex with a moiety capable of associating with said duplex, wherein said moiety is capable of effecting said signal;
- and
- (c) determining said sequence of said target nucleic acid by detecting an effect imparted by said moiety on said signal.
A method of genotyping a SNP, said method comprising:
- (a) mixing a first complex comprising a tracking component capable of generating a signal, and a first nucleic acid with a sample suspected of containing a second nucleic acid capable of forming a nucleic acid duplex with said first nucleic acid to form a second complex, wherein said second nucleic acid comprises said SNP;
- (b) contacting said second complex with a moiety capable of associating with said duplex, wherein said moiety is capable of effecting said signal;
- and
- (c) genotyping said SNP by detecting an effect imparted by said moiety on said signal.
A method of detecting a nucleic acid mutation, said method comprising:
- (a) mixing a first complex comprising a tracking component capable of generating a signal, and a first nucleic acid with a sample suspected of containing a second nucleic acid capable of forming a nucleic acid duplex with said first nucleic acid to form a second complex;
- (b) contacting said second complex with a moiety capable of associating with said duplex, wherein said moiety is capable of effecting said signal, wherein said second nucleic acid comprises said nucleic acid mutation;
- and
- (c) detecting said nucleic acid mutation by detecting an effect imparted by said moiety on said signal.
An in vivo method of determining the presence of a target agent, comprising:
- (a) administering a first complex comprising a tracking component capable of generating a signal, and a binding moiety capable of associating with said target agent, to a patient in a clinically-effective amount;
- (b) scanning said patient with a reader capable of detecting said signal;
- and
- (c) detecting said signal.
A composition comprising a tracking component, a biomolecule, and a metal-containing compound, wherein said metal-containing compound is a component of an antenna that affects a signal between said tracking component and a reader.
Dependencies are inferred from the claim text.