Analyzing the expression of biomarkers in cells with clusters

A system for analyzing tissue features based on multiplexed biometric image data comprising:

• a storage device for storing cell profile data comprising cell profile data including multiplexed biometric images capturing the expression of a plurality of biomarkers with respect to a field of view in which individual cells are delineated and segmenting into compartments;
• and
• at least one processor for executing code that causes the at least one processor to perform the steps of:
• assigning each of the cells in the field of view to a single cluster among a plurality of clusters of similar cells in a selected set of clusters, wherein each cluster in the selected set of clusters comprises cells having a plurality of selected attributes more similar to the plurality of selected attributes of other cells in that cluster than to the plurality of selected attributes of cells in other clusters in the set;
• observing a proportion of the cells assigned to each cluster in the selected set of clusters;
• and
• examining the observed proportions for an association with a diagnosis, a prognosis, or a response to treatment of a condition or a disease, wherein the association can be derived from a known association of the selected set of clusters with at least one piece of meta-information including a field of view level assessment or a patient-level assessment.