Method for dry packing chromatography columns
AssigneeGE Healthcare Bio-Sciences AB
InventorsKlaus Gebauer, Elisabeth Hallgren, Raf Lemmens, Ola Lind, Bjorn Lundgren, Jamil Shanagar, Kajsa Stridsberg-Friden, Helena Widegren
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Abstract
The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.
Claims30
A method for packing a chromatography column (1) with dry swellable particles comprising the steps of:
- a) transferring to a column chamber (2) in said chromatography column an amount of said dry swellable particles (5) sufficient to give a swollen volume Vs in a liquid of about 105-120% of the column chamber volume;
- b) closing the column;
- and
- c) providing said liquid to the column.
A column comprising dry swellable particles, wherein the amount of particles is sufficient to give a swollen volume Vs in an aqueous liquid of about 105-120% of the column chamber volume.
30-33. (canceled)
Dependencies are inferred from the claim text.