Method for Evaluating Inhibitory Polynucleotide Efficiency and Efficacy
AssigneeThe Trustees of Columbia University in the City of New York
InventorsIra S. Cohen, Thomas W. White, Richard B. Robinson, Peter R. Brink, Richard T. Mathias, Michael R. Rosen
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Abstract
The present invention provides a method for testing the efficiency of delivering an inhibitory polynucleotide to a target cell or tissue. The invention also provides a method for testing efficiency of delivering and efficacy for an effect on tumor size of an inhibitory polynucleotide against a target gene.
Claims34
A method for testing the efficiency of delivering a first inhibitory polynucleotide to a host animal, comprising (a) introducing target cells capable of forming a target cell mass and expressing a first fluorescent protein into the host animal at a target site;
- (b) delivering the first inhibitory polynucleotide against the first fluorescent protein to the host animal;
- and (c) measuring over a time period in vivo the first fluorescent protein fluorescence to determine the efficiency of delivering the first inhibitory polynucleotide to the target cells, wherein the efficiency is inversely proportional to a reduction in fluorescence.
The method of claim 27, wherein the first fluorescent protein and the target gene are expressed as a fusion protein in the target cells.
Dependencies are inferred from the claim text.