Scar-Less Multi-Part DNA Assembly Design Automation

A method of designing an implementation of a DNA assembly comprising:

• receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments;
• designing direct synthesis pieces and DNA oligonucleotides (oligos) for each of the DNA sequence fragments;
• and
• creating a plan for adding flanking homology sequences to each of the direct synthesis pieces.

A method of designing an implementation of a DNA assembly comprising:

• receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments;
• designing DNA oligonucleotides (oligos) and direct synthesis pieces for each of the DNA sequence fragments;
• and
• creating a plan for adding flanking homology sequences to each of the DNA oligos and to each of the direct synthesis pieces.

A method of designing an implementation of a DNA assembly comprising:

• receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments;
• designing DNA oligonucleotides (oligos) for each of e DNA sequence fragments;
• and
• creating a plan for adding optimized overhang sequences to each of the DNA oligos.

A method of designing an implementation of a DNA assembly comprising:

• receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments;
• designing direct synthesis pieces and DNA oligonucleotides (oligos) for each of the DNA sequence fragments;
• and
• creating a plan for adding optimized overhang sequences to each of the direct synthesis pieces.

A method of designing an implementation of a DNA assembly comprising:

• receiving a list of DNA sequence fragments to be assembled together and an order in which to assemble the DNA sequence fragments;
• designing DNA oligonucleotides (oligos) and direct synthesis pieces for each of the DNA sequence fragments;
• and
• creating a plan for adding optimized overhang sequences to each of the DNA oligos and to each of the direct synthesis pieces.