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US 2012/0259101 A1Published application
Isopeptide Bond Formation in Bacillus Species and Uses Thereof
OwnerLi Tan, Charles L. Turnbough, JR.inventors
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Abstract
A mechanism for a unique isopeptide bond formation between polypeptides is disclosed as well as sequence motifs used in such bond formation and methods of using such sequence motifs.
Claims25
A fusion protein, said fusion protein containing at least one donor sequence derived from a Bacillus species and a second polypeptide.
A fusion protein, said fusion protein containing at least one acceptor sequence derived from a Bacillus species and a second polypeptide.
A method linking one or more polypeptides through a covalent bond, the method comprising the steps of:
- a. providing a first polypeptide containing an acceptor sequence derived from a Bacillus species in a buffer;
- b. providing a second polypeptide containing a donor sequence derived from a Bacillus species;
- c. contacting the first and second polypeptides in the buffer in order to form a covalent bond between the acceptor sequence and the donor sequence, wherein the covalent bond is not a disulfide bond and the first polypeptide may optionally contain a donor sequence and the second polypeptide may optionally contain an acceptor sequence.
Dependencies are inferred from the claim text.