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US 2012/0259093 A1Published application
Expression of secreted human alpha-fetoprotein in transgenic animals
AssigneeMerrimack Pharmaceuticals, Inc.
InventorsStace LINDSAY, Robert Mulroy, Daniel Semeniuk
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Abstract
The invention features a process of expressing secreted recombinant human alpha-fetoprotein (rHuAFP) in the milk or urine of transgenic mammals.
Claims10
A substantially pure nucleic acid molecule comprising:
- (i) a nucleic acid sequence encoding recombinant human alpha-fetoprotein (rHuAFP), (ii) a milk-specific promoter, said promoter being operably linked to said rHuAFP-encoding sequence, and (iii) a leader sequence encoding a protein secretory signal that enables secretion of said rHuAFP by mile-producing cells into the milk of a mammal.
Non-human mammal's milk comprising biologically active recombinant human alpha-fetoprotein (rHuAFP).
A non-human transgenic mammal that expresses biologically active recombinant human alpha-fetoprotein (rHuAFP) in its milk, where in the genome of said mammal comprised a transgene that effects expression of rHuAFP in mammary epithelial cells of said mammal, wherein said transgene comprises:
- (i) a nucleic acid sequence encoding rHuAFP, (ii) a milk-specific promoter, said promoter being operably linked to said rHuAFP-encoding sequence, and (iii) a leader sequence encoding a protein secretory signal that enables secretion of said rHuAFP by said mammary epithelial cells into the milk of said mammal.
Dependencies are inferred from the claim text.