Agent that modulates physiological condition of pests, involved in insect peptidyl-dipeptidase a activity

A method for assaying pesticidal activity of a test substance, which comprises:

• (1) a first step of measuring the activity of a peptidyl-dipeptidase A selected from the following group A in a reaction system in which the peptidyl-dipeptidase A contacts with a test substance;
• and
• (2) a second step of evaluating the pesticidal activity of the test substance based on the difference obtained by comparing the activity measured in the first step with the activity of a control:
• <Group A>
• (a) a protein comprising the amino acid sequence of SEQ ID NO:
• 1;
• (b) a protein comprising an amino acid sequence with deletion, addition or substitution of one or more amino acids in the amino acid sequence of SEQ ID NO:
• 1, wherein the protein has peptidyl-dipeptidase A activity;
• (c) a protein comprising an amino acid sequence that has sequence identity of 65% or more to the amino acid sequence of SEQ ID NO. 1, wherein the protein has peptidyl-dipeptidase A activity;
• (d) a protein comprising the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 2;
• (e) a protein comprising an amino acid sequence encoded by a nucleotide sequence that has sequence identity of 65% or more to the nucleotide sequence of SEQ ID NO:
• 2, wherein the protein has peptidyl-dipeptidase A activity;
• (f) a protein comprising an amino acid sequence encoded by a polynucleotide, wherein the polynucleotide hybridizes under a stringent condition to a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
• 2, and wherein the protein has peptidyl-dipeptidase A activity;
• (g) a protein comprising an amino acid sequence of an insect peptidyl-dipeptidase A;
• and
• (h) a protein comprising an amino acid sequence of a cotton aphid peptidyl-dipeptidase A.

An insect peptidyl-dipeptidase A comprising an amino acid sequence selected from the following group B:

• <Group B>
• (a) the amino acid sequence of SEQ ID NO;
• 1;
• (b) an amino acid sequence with deletion, addition or substitution of one or more amino acids in the amino acid sequence of SEQ ID NO:
• 1, wherein the amino acid sequence has peptidyl-dipeptidase A activity;
• (c) an amino acid sequence that has sequence identity of 65% or more to the amino acid sequence of SEQ ID NO:
• 1, wherein the amino acid sequence has peptidyl-dipeptidase A activity;
• (d) the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO:
• 2;
• (e) an amino acid sequence encoded by a nucleotide sequence that has sequence identity of 65% or more to the nucleotide sequence of SEQ ID NO:
• 2, wherein the amino acid sequence has peptidyl-dipeptidase A activity;
• (f) an amino acid sequence encoded by a polynucleotide, wherein the polynucleotide hybridizes under a stringent condition to a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:
• 2, wherein the amino acid sequence has peptidyl-dipeptidase A activity;
• and
• (g) an amino acid sequence of a cotton aphid peptidyl-dipeptidase A.

A system which comprises:

• a means to input, store and manage a data information of an ability of test substances, wherein the ability is an ability to modulate the activity of an insect peptidyl-dipeptidase A;
• a means to query and retrieve the data information based on a desired criterion;
• and
• a means to display and output the result which is queried and retrieved.