Chromosomal landing pads and related uses

A recombinant polynucleotide for stably integrating a heterologous polynucleotide sequence into the genome of a mammalian cell, comprising:

• a first homology arm, the heterologous polynucleotide sequence, and a second homology arm;
• wherein the first and second homology arms are substantially identical to the 5′- and 3′-sequences, respectively, that flank a native chromosomal insertion site located within or adjacent to a gene selected from the group consisting of ankyrin 2 gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-1/Mal gene.

An engineered mammalian cell, comprising a heterologous polynucleotide which is stably integrated into its genome at one or more native chromosomal insertion sites located within or adjacent to a gene selected from the group consisting of ankyrin 2 gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene and Nephrocystin-1/Mal gene.

A method for stably integrating a heterologous polynucleotide into the genome of a mammalian cell, comprising:

• (a) inserting a site-specific recombination sequence into the genome of the cell, wherein the insertion is at a native chromosomal insertion site located within or adjacent to a gene selected from the group consisting of ankyrin 2 gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-1/Mal gene.
• (b) integrating by homologous recombination the heterologous polynucleotide into the genome of the cell at the inserted site-specific recombination sequence.