Protein-Free Culture Media Products for Manufacturing Viral-Based Vaccines

A substantially protein-free cell culture medium suitable for cultivation cell lines of mammalian and/or avian origins for manufacturing viral-based vaccines, comprising 0.1 g/L (0.0071 mM) methylcellulose having a molecular weight of 14,000 Daltons;

• 0.5 mM L-arginine;
• 0.01 mM L-cystine.2HCl;
• 0.02 mM L-histidine, 0.04 mM L-isoleucine;
• 0.04 mM L-leucine;
• 0.05 mM L-lysine.HCl;
• 0.01 mM L-methionine;
• 0.02 mM L-phenylalanine;
• 0.04 mM L-threonine;
• 0.005 mM L-tryptophan;
• 0.02 mM L-tyrosine.2Na2H2O;
• 0.04 mM L-valine;
• 0.5 mM L-alanine;
• 20 mM for L-taurine;
• 0.5 mM glutamic acid (0.39 mM);
• 20 mM L-glutamine or 0.25 mM L-Glycine;
• 3.1 mM (0.235 g/L) calcium chloride;
• 0.72 mM (0.087 g/L) magnesium sulfate;
• 4.8 mM (0.355 g/L) potassium chloride;
• 0.11 M (6.171 g/L) sodium chloride;
• 0.88 mM (0.108 g/L) for sodium dihydrogen phosphate;
• 0.004 mM (0.0005 g/L) choline chloride;
• 0.0024 mM (0.0006 g/L) D-biotin;
• 0.0067 mM (0.0012 g/L) myoinositol;
• 0.005 mM (0.0006 g/L) niacinamide;
• 0.0025 mM (0.0005 g/L) D-pantothenic acid;
• 0.003 (0.0006 g/L) pyridoxine HCl;
• 0.00016 mM (0.00006 g/L) riboflavin;
• 0.0018 mM (0.0005 g/L) thiamine HCl;
• 0.0014 mM (0.006 g/L) folic acid;
• 616 pM (800 ng/L) vitamin B12;
• 0.010 mM (0.004 g/L) vitamin E;
• between about 4.2 mM (0.75 g/L) to about 5.6 mM (1.0 g/L) D-glucose;
• 0.3 mM (0.02975 g/L) sodium pyruvate;
• 5.1 mM (0.92 g/L) fructose;
• 10 mM (1.13 g/L) sodium lactate;
• 0.3 mM (0.092 g/L) glutathione;
• 0.1 mM (0.0418 g/L) EDTA;
• 0.031 mM (0.011 g/L) phenol red;
• 2.8 micromolar D-mannitol;
• 26.2 mM (2.2 g/L) sodium bicarbonate;
• and 75 mg/L Penicillin G or from about 1.5 mg/L to about 4 mg/L gentamycin sulfate.