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US 2012/0258519 A1Published application
Protein Harvesting
AssigneeTherapeutic Proteins Inc.
InventorSarfaraz K. Niazi
Life of this patent
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Abstract
Methods of harvesting proteins directly from bioreactors to avoid at several steps in the purification of recombinant drugs are disclosed.
Claims68
A method of harvesting a target protein from a liquid in a first container comprising:
- A means of contacting said target protein with a resin capable of binding substantially all of said target protein to form a protein-resin conjugate;
- A means of separating said protein-resin conjugate from said liquid;
- A means of recovering said target protein from said protein-resin conjugate.
A method of purification of a recombinant protein in a culture media with host cells present at the end of a bioreaction cycle, said method comprising:
- Adding to said culture media a sufficient quantity of a resin suitable for the first stage of purification of said recombinant protein in a quantity capable of binding into a resin-protein conjugate, all or substantially all of said protein present in said culture media;
- Transferring said settled resin-protein conjugate to downstream purification column;
- Continuing with pre-established purification protocols of said purification column.
Dependencies are inferred from the claim text.