Cell-based systems for production of methyl formate
AssigneeThe Regents of the University of California
InventorsChristopher A. Voigt, Travis S. Bayer
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Abstract
Disclosed is a process in which a recombinant organism, such as a yeast, expressing a heterologous S-adenosylmethionme (SAM)-dependent methyl halide transferase (MHT) protein is combined with a halide and a carbon source in a cultivation medium under conditions in which methyl formate is produced. The cell may genetically modified to express methyl formate synthase, methanol dehydrogenase and/or hydrolytic dehalogenase at levels higher than a cell of the same species that is not genetically modified. The methyl formate may be collected and used in a variety of applications. The halide may be chlorine, bromine or iodine.
Claims25
A method comprising
- a) combining
- i) a recombinant organism, optionally a yeast, comprising a heterologous gene encoding S-adenosylmethionine (SAM)-dependent methyl halide transferase (MHT),
- ii) a halide selected from the group comprising chlorine, bromine and iodine;
- and
- iii) a carbon source;
- in a cultivation medium under conditions in which methyl formate is produced;
- and
- b) collecting methyl formate.
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A genetically engineered yeast cell expressing a heterologous Sadenosylmethionine (SAM)-dependent methyl halide transferase (MHT) and expressing one, two or three of:
- a) a heterologous methyl formate synthase,
- b) a heterologous methanol dehydrogenase,
- c) a heterologous hydrolytic dehalogenase.
A genetically engineered yeast cell expressing a heterologous Sadenosylmethionine (SAM)-dependent methyl halide transferase (MHT) and methyl formate synthase and/or methanol dehydrogenase and/or hydrolytic dehalogenase at levels higher than in a cell of the same species that is no so genetically modified.
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Dependencies are inferred from the claim text.