Biological synthesis of difunctional alkanes from carbohydrate feedstocks
AssigneeCelexion, LLC
InventorsBrian M. Baynes, John Michael Geremia
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Abstract
Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.
Claims2
A method of producing a α,ω difunctional Cn alkane from α-ketoglutarate wherein α and ω terminal functional groups are selected from the group of —OH, —COOH and —NH3 and wherein n is an integer in the range of 4 to 7 comprising:
- culturing a metabolically engineered host cell under conditions sufficient to produce the α,ω difunctional Cn alkane from a α-ketoacid, wherein the metabolically engineered host cell is genetically modified with a nucleic acid comprising at least one nucleotide sequence encoding at least two biosynthetic pathway enzymes;
- and
- separating said α,ω difunctional Cn alkane.
A method of producing a α,ω difunctional Cn alkane from α-ketoglutarate wherein α and ω terminal functional groups are selected from the group of —OH, —COOH and —NH3 and wherein n is an integer in the range of 5 to 8 comprising:
- culturing an engineered host cell under conditions sufficient to produce the α,ω difunctional Cn alkane, wherein the host cell comprises one or more exogenous nucleic acid sequences encoding at least two polypeptides selected from homocitrate synthase (EC 2.3.3.-), homoaconitase (EC 2.3.3.14), and homoisocitrate dehydrogenase (EC 1.1.1.-);
- and
- separating said α,ω difunctional Cn alkane.
Dependencies are inferred from the claim text.