Consolidated pretreatment and hydrolysis of plant biomass expressing cell wall degrading enzymes
AssigneeAgrivida, Inc.
InventorsR. Michael Raab, Dongcheng Zhang, Oleg Bougri
Life of this patent
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Abstract
Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided.
Claims49
A method for producing soluble sugars from engineered plant material comprising:
- pretreating by mixing the engineered plant material with a pulping formulation to form a mixture, wherein the engineered plant material includes a first polynucleotide sequence encoding a first protein selected from the group consisting of:
- a xylanase, an endoglucanase, an exoglucanase, a feruloyl esterase, an intein-modified xylanase, an intein-modified endoglucanase, an intein-modified exoglucanase, and an intein-modified feruloyl esterase;
- and
- providing hydrolysis conditions.
An engineered plant including a first polynucleotide sequence encoding an amino acid sequence with at least 90% identity to a first reference sequence selected from the group consisting of:
- SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, and SEQ ID NO:
- 11.
An engineered plant including at least one polynucleotide sequence encoding an amino acid sequence with at least 90% identity to a sequence selected from the group consisting of:
- SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 27, SEQ ID NO:
- 28, SEQ ID NO:
- 29, SEQ ID NO:
- 30, and SEQ ID NO:
- 31.
An expression cassette comprising:
- a first polynucleotide sequence capable of hybridizing under conditions of moderate stringency to a nucleic acid consisting of a first reference sequence selected from the group consisting of:
- SEQ ID NO:
- 32, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 37, SEQ ID NO:
- 38, and SEQ ID NO:
- 39;
- and a second polynucleotide sequence capable of hybridizing under conditions of moderate stringency to a nucleic acid consisting of a second reference sequence selected from the group consisting of:
- SEQ ID NO:
- 32, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 35, SEQ ID NO:
- 36, SEQ ID NO:
- 37, SEQ ID NO:
- 38, SEQ ID NO:
- 39, SEQ ID NO:
- 40, SEQ ID NO:
- 41, SEQ ID NO:
- 42, and SEQ ID:
- 43, wherein the SEQ ID NO selected as the first reference sequence is different than the SEQ ID NO selected as the second reference sequence.
An expression cassette comprising a polynucleotide sequence capable of hybridizing under conditions of moderate stringency to a nucleic acid consisting of a reference sequence selected from the group of sequences consisting of:
- SEQ ID NO:
- 52, SEQ ID NO:
- 53, SEQ ID NO:
- 54, SEQ ID NO:
- 55, SEQ ID NO:
- 56, SEQ ID NO:
- 57, SEQ ID NO:
- 58, SEQ ID NO:
- 59, SEQ ID NO:
- 60, SEQ ID NO:
- 61, and SEQ ID NO:
- 62.
An expression vector comprising a polynucleotide sequence capable of hybridizing under conditions of moderate stringency to nucleic acid consisting of a sequence selected from the group consisting of:
- SEQ ID NO:
- 68, SEQ ID NO:
- 69, SEQ ID NO:
- 70, SEQ ID NO:
- 71, SEQ ID NO:
- 72, SEQ ID NO:
- 73, SEQ ID NO:
- 74, SEQ ID NO:
- 75, SEQ ID NO:
- 76, SEQ ID NO:
- 77, SEQ ID NO:
- 78, SEQ ID NO:
- 79, SEQ ID NO:
- 80, SEQ ID NO:
- 81, SEQ ID NO:
- 82, and SEQ ID NO:
- 83.
Dependencies are inferred from the claim text.