Method of producing recombinant plasmid dna using substantially solid growth medium
OwnerVinod Pandiripally, Kunal Bisariyainventors
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Abstract
A method of producing recombinant plasmid DNA using substantially solid growth medium and disposable vessels in place of conventional liquid fermentation processes. The method includes inoculating a host organism containing the recombinant plasmid DNA onto the substantially solid growth medium in a disposable vessel; allowing the host organism to grow on the growth medium under conditions conducive to such growth; removing the host organism from the growth medium and lysing the host organism to access the recombinant plasmid DNA; and purifying the recombinant plasmid DNA.
Claims20
A method of large-scale production of recombinant plasmid DNA, the method comprising the steps of:
- inoculating a host organism containing the recombinant plasmid DNA onto a substantially solid growth medium in a disposable vessel;
- allowing the host organism to grow on the growth medium under conditions conducive to such growth;
- removing the host organism from the growth medium and lysing the host organism to access the recombinant plasmid DNA;
- and
- purifying the recombinant plasmid DNA.
A method of large-scale production of recombinant plasmid DNA including a desired gene sequence, the method comprising the steps of:
- obtaining a host organism containing the recombinant plasmid DNA including the desired gene sequence, wherein the host organism is a prokaryotic bacteria;
- inoculating the host organism onto a substantially solid growth medium in a disposable vessel;
- allowing the host organism to grow on the growth medium under conditions conducive to such growth;
- removing the host organism from the growth medium and lysing the host organism to access the recombinant plasmid DNA;
- and
- purifying the recombinant plasmid DNA.
A method of large-scale production of recombinant plasmid DNA including a desired gene sequence, the method comprising the steps of:
- obtaining a host organism containing the recombinant plasmid DNA including the desired gene sequence, wherein the host organism is a prokaryotic bacteria and the recombinant plasmid DNA contains an inducible replication origin and a promoter DNA sequence;
- inoculating the host organism onto a substantially solid growth medium in a disposable vessel;
- allowing the host organism to grow on the growth medium under conditions conducive to such growth;
- removing the host organism from the growth medium and lysing the host organism to access the recombinant plasmid DNA;
- and
- purifying the recombinant plasmid DNA.
Dependencies are inferred from the claim text.