Dna polymerases with improved activity
AssigneeRoche Molecular Systems, Inc.
InventorsKeith Bauer, Thomas Myers, Fred Reichert, Joseph San Filippo, Rachel Shahinian, Shawn Suko
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Abstract
Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
Claims20
A DNA polymerase having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors compared with a control DNA polymerase, wherein the amino acid of the DNA polymerase corresponding to position 709 of SEQ ID NO:1 is any amino acid other than I, L, or M, and wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase corresponding to position 709 of SEQ ID NO:1 is I, L, or M.
Dependencies are inferred from the claim text.