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US 2012/0258500 A1Published application
Pcr reaction mixtures with decreased non-specific activity
AssigneeBio-Rad Laboratories, Inc.
InventorMan Cheng
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Abstract
The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Claims21
A method of amplifying a nucleic acid molecule, the method comprising:
- (a) providing the nucleic acid in a composition sufficient for nucleic acid amplification, the composition comprising:
- (i) one or more oligonucleotide primers;
- (ii) a polymerase;
- and
- (iii) a sufficient amount of an agent to improve the specificity of nucleic acid amplification, wherein the agent comprises free arginine, spermidine, or spermine, or a salt thereof;
- and
- (b) incubating the mixture under conditions sufficient for amplifying the nucleic acid, thereby amplifying the nucleic acid.
4 more elements
A reaction mixture comprising:
- a polymerase;
- and
- a sufficient amount of an agent to improve the specificity of nucleic acid amplification, wherein the agent comprises free arginine, spermidine, or spermine, or a salt thereof.
A kit comprising:
- a polymerase;
- and
- a sufficient amount of an agent to improve the specificity of nucleic acid amplification, wherein the agent comprises free arginine, spermidine, or spermine, or a salt thereof.
Dependencies are inferred from the claim text.