Retroviral Vector Particles and Methods for their Generation and Use
Assignee4-Anitbody AG
InventorJörn Stitz
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Abstract
The present invention relates to methods of host cell transduction utilising ecotropic retroviral vector particles. The retroviral vector particle may comprise an envelope of Friend murine leukaemia virus, in particular the envelope encoded by molecular clone PVC-211 and the host cell may be engineered to recombinantly express the Reel receptor. The retroviral vector particles and methods of the invention can be used to introduce expressible polynucleotide sequences of interest into host cells with high efficiency. This results in protein production methods with higher yield (mg/L) and a reduction in manufacturing costs that could be used in a range of applications including for example, the production of therapeutic proteins, vaccines and antibodies.
Claims22
A method for transducing a host cell, said method comprising transducing the host cell with a retroviral vector particle comprising an envelope of Friend murine leukaemia virus, wherein the envelope of Friend murine leukaemia virus is encoded by molecular clone PVC-211 (SEQ ID NO:
- 1) or a functional fragment thereof, and wherein the host cell recombinantly expresses a Rec1 receptor (SEQ ID No:
- 32) or a functional fragment thereof.
A method for conferring or increasing susceptibility of a host cell to transduction, said method comprising transducing the host cell with a retroviral vector particle comprising an envelope of Friend murine leukaemia virus, wherein the envelope of Friend murine leukaemia virus is encoded by molecular clone PVC-211 (SEQ ID NO:
- 1) or a functional fragment thereof, and wherein the host cell recombinantly expresses a Rec1 receptor (SEQ ID No:
- 32) or a functional fragment thereof.
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A host cell line transduced with a retroviral vector particle comprising an envelope of Friend murine leukaemia virus encoded by the molecular clone PVC-211 (SEQ ID No:
- 1) or a functional fragment thereof, wherein the host cell line recombinantly expresses a Rec1 receptor.
(canceled)
A system for transducing a host cell, comprising:
- a) a retroviral vector particle comprising an envelope of Friend murine leukaemia virus encoded by the molecular clone PVC-211 (SEQ ID No:
- 1) or a functional fragment thereof;
- and
- b) a target host cell which recombinantly expresses a Rec1 receptor (SEQ ID No:
- 32) or a functional fragment thereof.
Dependencies are inferred from the claim text.