Calcium-binding photoprotein, gene encoding the same, and use thereof
AssigneeJnc Corporation
InventorsSatoshi INOUYE, Yuiko SAHARA
Life of this patent
Estimating the term…
Abstract
A protein according to the invention can be used to detect or measure calcium ions is provided. Further the protein is useful as a reporter protein or a luminescence marker. A polynucleotide according to the invention is also useful as a reporter gene.
Claims7
1-18. (canceled)
A method for detecting or assaying calcium ions, comprising the use of a protein comprising at least one item (a) and (b):
- (a) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and
- (b) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and having the ability to bond with a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion, or
- a holoprotein comprising said protein;
- and
- at least one of a peroxide of coelenterazine and a peroxide of a coelenterazine derivative.
A method for measuring activity of a sequence which participates in promoter control, comprising the use of a polynucleotide encoding a protein comprising at least one items (a) and (b):
- (a) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and
- (b) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and having the ability to bond with a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion;
- as a reporter gene.
A method for measuring changes in intracellular calcium concentration, comprising a step of inducing expression of a polynucleotide encoding a protein comprising at least one item (a) and (b):
- (a) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and
- (b) a protein comprising an amino acid sequence selected from the group consisting of an amino acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, SEQ ID NO:
- 9 and SEQ ID NO:
- 11, and having the ability to bond with a peroxide of coelenterazine or a peroxide of a coelenterazine derivative so as to form a holoprotein that emits light under the action of a calcium ion;
- within a cell so as to form a photoprotein.
Dependencies are inferred from the claim text.