Cancer diagnosis marker using the aberrant glycosylation of a protein

A method for quantitative analysis of polypeptide to provide information for cancer diagnosis comprising the following steps:

• 1) separating and concentrating glycoproteins by treating a sample obtained from a subject with lectin;
• 2) hydrolyzing the glycoproteins of step 1) to obtain polypeptides;
• 3) quantitatively analyzing the polypeptides of step 2);
• and
• 4) diagnosing cancer or high risk of cancer when the subject has at least one of those polypeptides selected from the group consisting of those polypeptides having the molecular weights of 2294.1, 2015.0, 1685.8, 1346.7, 1265.7 and 1109.5 confirmed by the quantitative analysis of step 3).

A method for sequencing of polypeptide to provide information for cancer diagnosis comprising the following steps:

• 1) separating and concentrating glycoproteins by treating a sample obtained from a subject with lectin;
• 2) hydrolyzing the glycoproteins of step 1) to obtain polypeptides;
• 3) sequencing the polypeptides of step 2);
• and
• 4) diagnosing cancer or high risk of cancer when the subject has at least one of those polypeptides selected from the group consisting of those polypeptides having the amino acid sequences represented by SEQ. ID. NO:
• 1-NO:
• 6 confirmed by the sequencing of step 3).

A method for quantitative analysis and sequencing of polypeptide to provide information for cancer diagnosis comprising the following steps:

• 1) separating and concentrating glycoproteins by treating a sample obtained from a subject with lectin;
• 2) hydrolyzing the glycoproteins of step 1) to obtain polypeptides;
• 3) sequencing and quantitatively analyzing the polypeptides of step 2);
• and
• 4) diagnosing cancer or high risk of cancer when the subject has at least one of those polypeptides confirmed by the sequencing and quantitative analysis of step 3), wherein the polypeptide is selected from the group consisting of those polypeptides having the amino acid sequences represented by SEQ. ID. NO:
• 1-NO:
• 6 and selected from the group consisting of those polypeptides having the molecular weights of 2294.1, 2015.0, 1685.8, 1346.7, 1265.7 and 1109.5.