Cross-linking atp analogs
AssigneeWayne State University
InventorMary Kay Pflum
Life of this patent
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Abstract
An adenosine 5′-triphosphate analogs modified at the gamma-phosphate with a reactive reagent. A method of forming the analog by activating a 4-amino benzoic acid, incubating the activated acid to obtain an amine, and coupling the amine with ATP in the presence of water soluble EDCI. A method of detecting the efficacy of a therapeutic by adding a gamma-phosphate modified ATP analog to a protein substrate, reacting the target proteins with the ATP analog, and analyzing the resulting cross-linked product, wherein the amount of product present correlates to the efficacy of the therapeutic is also provided.
Claims15
An analog comprising adenosine 5′-triphosphate analogs modified at the gamma-phosphate with a reactive reagent.
A kit for detecting characterizing a target protein in a sample, the kit comprising:
- a gamma-phosphate modified ATP analog for selectively tagging a protein at phosphorylated amino acids with a cross-linking group.
A method of detecting the efficacy of a therapeutic comprising the steps of:
- adding a gamma-phosphate modified ATP analog to a protein substrate;
- reacting the target proteins with the ATP analog;
- and
- analyzing the resulting cross-linked product, wherein the amount of product present correlates to the efficacy of the therapeutic.
Dependencies are inferred from the claim text.