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US 2012/0258474 A1Published application
Methods of detecting dna damage
AssigneeGe Healthcare UK Limited
InventorsPeter James Tatnell, Jeffrey Kenneth Horton, Nicholas Thomas
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Abstract
The present invention relates to methods of detecting agents that cause or may potentiate DNA damage, and to assays that may be employed in such methods. In particular, the invention relates to methods of detecting DNA damage in in vitro cultures of human cells.
Claims16
An in vitro method of measuring the genotoxicity of an agent in human cells comprising the steps of:
- (i) providing an in vitro culture of human cells comprising the phosphatidylinositol glycan biosynthesis class A (PigA) gene and expressing at least one glycosylphosphatidylinositol (GPI) anchor;
- (ii) determining a control level of said GPI-anchor or a GPI-anchored protein in said cells;
- (iii) exposing the cells to an agent;
- (iv) determining a treatment level of the GPI-anchor or said GPI-anchored protein;
- (v) comparing said treatment level with said control level of the GPI-anchor or the GPI-anchored protein,
- wherein a difference in the levels of the GPI-anchor or the GPI-anchored protein is indicative of the genotoxicity of said agent.
3 more elements
16-20. (canceled)
Dependencies are inferred from the claim text.