Laser isolation of viable cells
AssigneeAdvanced Cell Technology, Inc.
InventorIrina Vitaly KLIMANSKAYA
Life of this patent
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Abstract
Methods for laser microdissection isolation of viable cells are provided. Cells of a desired type may be isolated from a diverse population, optionally with detection and exclusion of undesired cells. Desired cells may be isolated from a population that arose from differentiation of pluripotent cells, preferably embryonic stem cells or induced pluripotent stem cells, and undifferentiated stem cells may be detected and excluded from selection including the isolation of RPE cells sleeted based on morphology (e.g., characteristic mottled appearance) from a population of ES cells. The cells isolated by these methods, including RPE cells, may be essentially free of undifferentiated cells and thus suitable for use in cell-based therapies.
Claims32
1-66. (canceled)
A method for isolating a viable cell from a heterogeneous population of cells comprising
- (a) providing a planar carrier on which said population of cells containing said at least one viable cell is situated,
- (b) placing said culture dish in a microscope coupled to a laser microdissection system,
- (a) selecting said viable cell,
- (b) excising said viable cell,
- (c) separating said viable cell from the planar carrier, and
- (d) collecting said viable cell.
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89-95. (canceled)
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A method for isolating a RPE cell from a population of cells comprising
- (a) providing a planar carrier on which said population of cells is situated,
- (b) placing said planar carrier in a microscope coupled to a laser microdissection system,
- (c) selecting said at least one RPE cell,
- (d) excising said cell from undesired cells or other materials in target areas adjacent to the selected cells using laser light, thereby severing the connections between the selected cells and adjacent cells or other materials, and
- (e) collecting said RPE cell.
106-138. (canceled)
A method of isolating a viable RPE cell from a heterogeneous population of cells comprising
- (a) providing a planar carrier on which a cell population comprising at least one viable desired cell is situated;
- (b) selecting at least one desired cell to be isolated;
- (c) excising said at least one cell from undesired cells or other materials in target areas adjacent to the selected cells using laser light, thereby severing the connections between the selected cells and adjacent cells or other materials;
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- (d) separating the at least one selected cell from the planar carrier, thereby isolating the selected cells, wherein the isolated cells comprise viable desired cells, wherein said desired cells are of a desired cell type selected from the group consisting of iris pigment epithelium cells, vision-associated neural cells, lens cells, rod cells, cone cells, or corneal cells.
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Dependencies are inferred from the claim text.