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AssigneeReceptomon, LLC
InventorJoseph F. Norfray
Estimating the term…
Disclosed is a method for monitoring change in gene expression of translation and integral/secretory protein synthesis in a sample comprising measuring the amount of Choline and/or a fatty acid present in the sample as a function of time wherein the measurement is carried out by Magnetic Resonance Spectroscopy (MRS) and correlating the amount of Choline and/or fatty acid measured as function of time to the change in gene expression of translation and integral/secretory protein synthesis in the sample. The level of Choline from organelles matches the level of Choline from transcription and integral/secretory protein synthesis. Quantification of translation and integral/secretory protein synthesis leads to quantification of genetic expression. During apoptosis the amount of Choline decreases, and the amount of fatty acids increase from distinct trafficking of Choline polar heads and fatty and tails of degraded endomembranes.
A method for monitoring change in gene expression of translation and integral/secretory protein synthesis in a sample comprising measuring the amount of Choline and/or a fatty acid present in the sample as a function of time wherein the measurement is carried out by Magnetic Resonance Spectroscopy (MRS) and correlating the amount of Choline and/or fatty acid measured as function of time to the change in gene expression of translation and integral/secretory protein synthesis in the sample.
A method for monitoring gene expression comprising:
A method for monitoring translation and integral/secretory protein synthesis in a tissue comprising and measuring, by Magnetic Resonance Spectroscopy, the amount of Choline present in the intracellular membranes of the tissue initially and after an interval, obtaining a difference between the amounts Choline, and correlating the change to changes of protein translation and integral/secretory protein synthesis.
A method for quantifying the genetic pathways of apoptosis, autophagy and unfolded protein response by measuring non-invasively, by Magnetic Resonance Spectroscopy, the amount of Choline present in the tissue before and within a period of about 168 hours, obtaining a difference between the Choline amounts, and correlating the changes in the Choline amounts with changes in genetic pathways.
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