Method for characterizing host immune funtion by ex vivo induction of offensive and defensive immune markers
AssigneesHitachi Chemical Research Center, Inc., Hitachi Chemical Company, Ltd.
InventorMasato Mitsuhashi
Life of this patent
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Abstract
A host's immune function can be characterized by quantifying changes in offensive and defensive immune function associated markers. Certain methods can be used to identify a potentially efficacious therapy for a subject based on the induction of expression of offensive and defensive immune function-associated markers. Additionally, some methods can be used to identify drugs that allow the stimulation of either the offensive or defensive immune response while inhibiting the other of offensive or defensive immune response.
Claims21
-34. (canceled)
A method for enabling a medical professional to recommend an immune-based or non-immune-based therapy to a subject, the method comprising:
- obtaining at least a first and a second aliquot of whole blood from the subject;
- exposing the first aliquot to a solvent and exposing the second aliquot to the solvent further comprising an immune cell stimulating agent;
- quantifying an amount of mRNA encoding an immune cell function-related mRNA in each of the first and second aliquots;
- calculating a ratio of the amount the immune cell function-related mRNA in the first aliquot to the amount of immune cell function-related mRNA in the second aliquot;
- and
- 1) indicating to a medical professional whether the ratio is less than 1 or greater than 1 so as to enable the medical professional to recommend an immune-based therapy if the ratio is less than 1 and a non-immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is IGH2, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is CD16;
- or
- 2) indicating to a medical professional whether the ratio is less than 1 or greater than 1 so as to enable the medical professional to recommend an non-immune-based therapy if the ratio is less than 1 and an immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is recombinant interleukin-2 (rIL2) and the immune cell function-related mRNA is FOXP3, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is arginase 1.
A method for advising a therapy to a subject based on the subject's immune cell function function, the method comprising:
- ordering a test of the subject's blood, said test comprising:
- obtaining at least a first and a second aliquot of heparinized whole blood from the subject;
- exposing the first aliquot to a solvent and exposing the second aliquot to the solvent further comprising an immune cell stimulating agent;
- quantifying an amount of mRNA encoding an immune cell function-related mRNA in each of the first and second aliquots;
- calculating a ratio of the amount the immune cell function-related mRNA in the first aliquot to the amount of immune cell function-related mRNA in the second aliquot;
- and
- 1) advising the subject to undergo an immune-based therapy if the ratio is less than 1 and a non-immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is IGH2, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is CD16;
- or
- 2) advising the subject to undergo a non-immune-based therapy if the ratio is less than 1 and an immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is recombinant interleukin-2 (rIL2) and the immune cell function-related mRNA is FOXP3, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is arginase 1.
A method for treating a subject having cancer based on the subject's regulatory immune cell function, the method comprising:
- ordering a test of the subject's blood, said test comprising:
- obtaining at least a first and a second aliquot of heparinized whole blood from the subject;
- exposing the first aliquot to a solvent and exposing the second aliquot to the solvent further comprising an immune cell stimulating agent;
- quantifying an amount of mRNA encoding an immune cell function-related mRNA in each of the first and second aliquots;
- calculating a ratio of the amount the immune cell function-related mRNA in the first aliquot to the amount of immune cell function-related mRNA in the second aliquot;
- and
- 1) administering to the an immune-based therapy if the ratio is less than 1 and a non-immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is IGH2, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is CD16;
- or
- 2) administering to the subject a non-immune-based therapy if the ratio is less than 1 and an immune-based therapy if the ratio is greater than 1,
- a) wherein the immune stimulating agent is recombinant interleukin-2 (rIL2) and the immune cell function-related mRNA is FOXP3, or
- b) wherein the immune stimulating agent is zymosan A and the immune cell function-related mRNA is arginase 1.
Dependencies are inferred from the claim text.